Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction

Zachary L. Newman, Adam Hoagland, Krishan Aghi, Kurtresha Worden, Sabrina L. Levy, Jun Ho Son, Luke P. Lee, Ehud Y. Isacoff

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Synaptic connections undergo activity-dependent plasticity during development and learning, as well as homeostatic re-adjustment to ensure stability. Little is known about the relationship between these processes, particularly in vivo. We addressed this with novel quantal resolution imaging of transmission during locomotive behavior at glutamatergic synapses of the Drosophila larval neuromuscular junction. We find that two motor input types, Ib and Is, provide distinct forms of excitatory drive during crawling and differ in key transmission properties. Although both inputs vary in transmission probability, active Is synapses are more reliable. High-frequency firing “wakes up” silent Ib synapses and depresses Is synapses. Strikingly, homeostatic compensation in presynaptic strength only occurs at Ib synapses. This specialization is associated with distinct regulation of postsynaptic CaMKII. Thus, basal synaptic strength, short-term plasticity, and homeostasis are determined input-specifically, generating a functional diversity that sculpts excitatory transmission and behavioral function.

Original languageEnglish
Pages (from-to)1388-1404.e10
JournalNeuron
Volume93
Issue number6
DOIs
StatePublished - 22 Mar 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • CaMKII
  • Drosophila melanogaster
  • homeostatic plasticity
  • input-specific plasticity
  • neuromuscular junction
  • optical quantal analysis
  • release probability
  • short-term plasticity

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