Injectable thermogel for 3D culture of stem cells

Madhumita Patel; Hyun Jung Lee; Sohee Park; Yelin Kim; Byeongmoon Jeong

doi:10.1016/j.biomaterials.2018.01.001

Injectable thermogel for 3D culture of stem cells

Madhumita Patel, Hyun Jung Lee, Sohee Park, Yelin Kim, Byeongmoon Jeong

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Review article › peer-review

84 Scopus citations

Abstract

Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells. This review focuses on the crucial questions that need to be addressed to achieve effective differentiation of stem cells into target cells, comprising low modulus, cell adhesion, and controlled supply of the growth factors. Recent progress in the use of thermogel as a 3D culture system of stem cells is summarized, and our perspectives on designing a new thermogel for 3D culture and its eventual application to injectable tissue engineering of stem cells are presented.

Original language	English
Pages (from-to)	91-107
Number of pages	17
Journal	Biomaterials
Volume	159
DOIs	https://doi.org/10.1016/j.biomaterials.2018.01.001
State	Published - Mar 2018

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government ( 2017R1A2B2007356 , 2017R1A5A1015365 , and 2014M3A9B6034223 ). MP and HJL equally contributed to this paper. All authors have given approval to the final version of the manuscript.

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

3D culture
Stem cell
Temperature-sensitivity
Thermogel
Tissue engineering

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10.1016/j.biomaterials.2018.01.001

Cite this

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title = "Injectable thermogel for 3D culture of stem cells",

abstract = "Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells. This review focuses on the crucial questions that need to be addressed to achieve effective differentiation of stem cells into target cells, comprising low modulus, cell adhesion, and controlled supply of the growth factors. Recent progress in the use of thermogel as a 3D culture system of stem cells is summarized, and our perspectives on designing a new thermogel for 3D culture and its eventual application to injectable tissue engineering of stem cells are presented.",

keywords = "3D culture, Stem cell, Temperature-sensitivity, Thermogel, Tissue engineering",

author = "Madhumita Patel and Lee, {Hyun Jung} and Sohee Park and Yelin Kim and Byeongmoon Jeong",

note = "Funding Information: This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government ( 2017R1A2B2007356 , 2017R1A5A1015365 , and 2014M3A9B6034223 ). MP and HJL equally contributed to this paper. All authors have given approval to the final version of the manuscript. Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

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doi = "10.1016/j.biomaterials.2018.01.001",

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AU - Lee, Hyun Jung

AU - Park, Sohee

AU - Kim, Yelin

AU - Jeong, Byeongmoon

N1 - Funding Information: This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government ( 2017R1A2B2007356 , 2017R1A5A1015365 , and 2014M3A9B6034223 ). MP and HJL equally contributed to this paper. All authors have given approval to the final version of the manuscript. Publisher Copyright: © 2018 Elsevier Ltd

PY - 2018/3

Y1 - 2018/3

N2 - Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells. This review focuses on the crucial questions that need to be addressed to achieve effective differentiation of stem cells into target cells, comprising low modulus, cell adhesion, and controlled supply of the growth factors. Recent progress in the use of thermogel as a 3D culture system of stem cells is summarized, and our perspectives on designing a new thermogel for 3D culture and its eventual application to injectable tissue engineering of stem cells are presented.

AB - Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells. This review focuses on the crucial questions that need to be addressed to achieve effective differentiation of stem cells into target cells, comprising low modulus, cell adhesion, and controlled supply of the growth factors. Recent progress in the use of thermogel as a 3D culture system of stem cells is summarized, and our perspectives on designing a new thermogel for 3D culture and its eventual application to injectable tissue engineering of stem cells are presented.

KW - 3D culture

KW - Stem cell

KW - Temperature-sensitivity

KW - Thermogel

KW - Tissue engineering

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DO - 10.1016/j.biomaterials.2018.01.001

M3 - Review article

C2 - 29316455

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SN - 0142-9612

VL - 159

SP - 91

EP - 107

JO - Biomaterials

JF - Biomaterials

ER -

Injectable thermogel for 3D culture of stem cells

Abstract

Bibliographical note

Keywords

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