Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo

Jue Yeon Lee; Jung Eun Choo; Hyun Jung Park; Jun Bum Park; Sang Chul Lee; Inho Jo; Seung Jin Lee; Chong Pyoung Chung; Yoon Jeong Park

doi:10.1016/j.bbrc.2007.03.106

Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo

Jue Yeon Lee, Jung Eun Choo, Hyun Jung Park, Jun Bum Park, Sang Chul Lee, Inho Jo, Seung Jin Lee, Chong Pyoung Chung, Yoon Jeong Park

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A synthetic peptide denoted as collagen-binding motif (CBM) was identified from osteopontin (OPN), a multisubunit extracellular matrix (ECM) protein, by enzymatic digestion with chymotrypsin. The aim of this study was to examine the feasibility of identified CBM peptide as an active component of gel type scaffold material in osteogenesis. The binding of CBM peptide to collagen was specific and presented high affinity. Cell adhesion and growth on CBM peptide-immobilized gel were significantly increased as compared with those on gel with control peptide or without peptide. The CBM peptide-immobilized gel increased osteoblastic differentiation, followed by marked bone formation in the rabbit calvarial defect sites at 4 weeks. Taken together, the injectable gel with synthetic CBM peptide has a potential to induce osteogenesis in vitro and in vivo, suggesting its clinical application in bone regeneration procedure.

Original language	English
Pages (from-to)	68-74
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2007.03.106
State	Published - 25 May 2007

Bibliographical note

Funding Information:
This study was supported in part by Korea Science and Engineering Foundation (KOSEF) Nanobiotechnology development program (#2006-00952) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.

Keywords

Alginate
Collagen-binding motif
Immobilization
Injectable gel
Osteoblastic differentiation
Osteogenesis
Osteopontin

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title = "Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo",

abstract = "A synthetic peptide denoted as collagen-binding motif (CBM) was identified from osteopontin (OPN), a multisubunit extracellular matrix (ECM) protein, by enzymatic digestion with chymotrypsin. The aim of this study was to examine the feasibility of identified CBM peptide as an active component of gel type scaffold material in osteogenesis. The binding of CBM peptide to collagen was specific and presented high affinity. Cell adhesion and growth on CBM peptide-immobilized gel were significantly increased as compared with those on gel with control peptide or without peptide. The CBM peptide-immobilized gel increased osteoblastic differentiation, followed by marked bone formation in the rabbit calvarial defect sites at 4 weeks. Taken together, the injectable gel with synthetic CBM peptide has a potential to induce osteogenesis in vitro and in vivo, suggesting its clinical application in bone regeneration procedure.",

keywords = "Alginate, Collagen-binding motif, Immobilization, Injectable gel, Osteoblastic differentiation, Osteogenesis, Osteopontin",

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note = "Funding Information: This study was supported in part by Korea Science and Engineering Foundation (KOSEF) Nanobiotechnology development program (#2006-00952) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea. ",

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AU - Lee, Jue Yeon

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AU - Lee, Sang Chul

AU - Jo, Inho

AU - Lee, Seung Jin

AU - Chung, Chong Pyoung

AU - Park, Yoon Jeong

N1 - Funding Information: This study was supported in part by Korea Science and Engineering Foundation (KOSEF) Nanobiotechnology development program (#2006-00952) entitled by Regenomics (Innovative surface activation of regenerative biomaterials) and in part by Engineering Research Center grant from KOSEF through Intelligent Biointerface Engineering Center (IBEC), Seoul National University, Korea.

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N2 - A synthetic peptide denoted as collagen-binding motif (CBM) was identified from osteopontin (OPN), a multisubunit extracellular matrix (ECM) protein, by enzymatic digestion with chymotrypsin. The aim of this study was to examine the feasibility of identified CBM peptide as an active component of gel type scaffold material in osteogenesis. The binding of CBM peptide to collagen was specific and presented high affinity. Cell adhesion and growth on CBM peptide-immobilized gel were significantly increased as compared with those on gel with control peptide or without peptide. The CBM peptide-immobilized gel increased osteoblastic differentiation, followed by marked bone formation in the rabbit calvarial defect sites at 4 weeks. Taken together, the injectable gel with synthetic CBM peptide has a potential to induce osteogenesis in vitro and in vivo, suggesting its clinical application in bone regeneration procedure.

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Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo

Abstract

Bibliographical note

Keywords

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