Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo

Jue Yeon Lee, Jung Eun Choo, Hyun Jung Park, Jun Bum Park, Sang Chul Lee, Inho Jo, Seung Jin Lee, Chong Pyoung Chung, Yoon Jeong Park

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

A synthetic peptide denoted as collagen-binding motif (CBM) was identified from osteopontin (OPN), a multisubunit extracellular matrix (ECM) protein, by enzymatic digestion with chymotrypsin. The aim of this study was to examine the feasibility of identified CBM peptide as an active component of gel type scaffold material in osteogenesis. The binding of CBM peptide to collagen was specific and presented high affinity. Cell adhesion and growth on CBM peptide-immobilized gel were significantly increased as compared with those on gel with control peptide or without peptide. The CBM peptide-immobilized gel increased osteoblastic differentiation, followed by marked bone formation in the rabbit calvarial defect sites at 4 weeks. Taken together, the injectable gel with synthetic CBM peptide has a potential to induce osteogenesis in vitro and in vivo, suggesting its clinical application in bone regeneration procedure.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number1
DOIs
StatePublished - 25 May 2007

Keywords

  • Alginate
  • Collagen-binding motif
  • Immobilization
  • Injectable gel
  • Osteoblastic differentiation
  • Osteogenesis
  • Osteopontin

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