Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

Kyoung Ah Kang; Rui Zhang; Mei Jing Piao; Kyoung Hwa Lee; Bum Joon Kim; So Young Kim; Hee Sun Kim; Dong Hyun Kim; Ho Jin You; Jin Won Hyun

doi:10.1080/10715760701241618

Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

Kyoung Ah Kang, Rui Zhang, Mei Jing Piao, Kyoung Hwa Lee, Bum Joon Kim, So Young Kim, Hee Sun Kim, Dong Hyun Kim, Ho Jin You, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H₂O₂) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H₂O₂ treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H₂O₂ induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.

Original language	English
Pages (from-to)	720-729
Number of pages	10
Journal	Free Radical Research
Volume	41
Issue number	6
DOIs	https://doi.org/10.1080/10715760701241618
State	Published - 2007

Keywords

Cytoprotective properties
Glycitein
Oxidative stress
Reactive oxygen species

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@article{842bdd67dead42efbe06142466b87b64,

title = "Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase",

abstract = "The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H2O2 treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H2O2 induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.",

keywords = "Cytoprotective properties, Glycitein, Oxidative stress, Reactive oxygen species",

author = "Kang, {Kyoung Ah} and Rui Zhang and Piao, {Mei Jing} and Lee, {Kyoung Hwa} and Kim, {Bum Joon} and Kim, {So Young} and Kim, {Hee Sun} and Kim, {Dong Hyun} and You, {Ho Jin} and Hyun, {Jin Won}",

note = "Funding Information: This research was supported by the study of the DNA repair regulation with disease program and by the program of Basic Atomic Energy Research Institute (BAERI) which is a part of the Nuclear R&D programs grant from the Ministry of Science and Technology of Korea.",

year = "2007",

doi = "10.1080/10715760701241618",

language = "English",

volume = "41",

pages = "720--729",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "6",

}

TY - JOUR

T1 - Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

AU - Kang, Kyoung Ah

AU - Zhang, Rui

AU - Piao, Mei Jing

AU - Lee, Kyoung Hwa

AU - Kim, Bum Joon

AU - Kim, So Young

AU - Kim, Hee Sun

AU - Kim, Dong Hyun

AU - You, Ho Jin

AU - Hyun, Jin Won

N1 - Funding Information: This research was supported by the study of the DNA repair regulation with disease program and by the program of Basic Atomic Energy Research Institute (BAERI) which is a part of the Nuclear R&D programs grant from the Ministry of Science and Technology of Korea.

PY - 2007

Y1 - 2007

N2 - The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H2O2 treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H2O2 induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.

AB - The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H2O2 treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H2O2 induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.

KW - Cytoprotective properties

KW - Glycitein

KW - Oxidative stress

KW - Reactive oxygen species

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U2 - 10.1080/10715760701241618

DO - 10.1080/10715760701241618

M3 - Article

C2 - 17516245

AN - SCOPUS:34249824273

SN - 1071-5762

VL - 41

SP - 720

EP - 729

JO - Free Radical Research

JF - Free Radical Research

IS - 6

ER -

Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

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