Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

Kyoung Ah Kang, Rui Zhang, Mei Jing Piao, Kyoung Hwa Lee, Bum Joon Kim, So Young Kim, Hee Sun Kim, Dong Hyun Kim, Ho Jin You, Jin Won Hyun

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H2O2 treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H2O2 induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.

Original languageEnglish
Pages (from-to)720-729
Number of pages10
JournalFree Radical Research
Volume41
Issue number6
DOIs
StatePublished - 2007

Keywords

  • Cytoprotective properties
  • Glycitein
  • Oxidative stress
  • Reactive oxygen species

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