Inhibitory effects of epigallocatechin-3-O-gallate on serum-stimulated rat aortic smooth muscle cells via nuclear factor-κB down-modulation

Dong Wook Han, Hye Ryeon Lim, Hyun Sook Baek, Mi Hee Lee, Seung Jin Lee, Suong Hyu Hyon, Jong Chul Park

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


The abnormal growth of vascular smooth muscle cells (VSMCs) plays an important role in vascular diseases, including atherosclerosis and restenosis after angioplasty. Although (-)-epigallocatechin-3-O-gallate (EGCG) has antiproliferative effects on various cells, relatively a little is known about precise mechanisms of the inhibitory effects of EGCG on SMCs. In this study, the inhibitory effects of EGCG on attachment, proliferation, migration, and cell cycle of rat aortic SMCs (RASMCs) with serum stimulation were investigated. Also, the involvement of nuclear factor-κB (NF-κB) during these inhibitions by EGCG was examined. EGCG treatment resulted in significant (p < 0.05) inhibition in attachment and proliferation of RASMCs induced by serum. While non-treated RASMCs migrated into denuded area in response to serum and showed essentially complete closure after 36 h, EGCG-treated cells covered only 31% of the area even after 48 h of incubation. Furthermore, EGCG treatment resulted in an appreciable cell cycle arrest at both G0/G1- and G2/M-phases. The immunoblot analysis revealed that the constitutive expression of NF-κB/p65 nuclear protein in RASMCs was lowered by EGCG in both the cytosol and the nucleus in a dose-dependent manner. These results suggest that the EGCG-caused inhibitory effects on RASMCs may be mediated through NF-κB down-modulation.

Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - 23 Jun 2006

Bibliographical note

Funding Information:
This work was supported by the NanoBio R&D Program [Platform technologies for organ/tissue regeneration (Regenomics), Grant No. 2005-00009] of the Korea Science and Engineering Foundation.


  • Antiproliferation
  • Cell cycle arrest
  • Epigallocatechin-3-O-gallate
  • Nuclear factor-κB
  • Rat aortic smooth muscle cell


Dive into the research topics of 'Inhibitory effects of epigallocatechin-3-O-gallate on serum-stimulated rat aortic smooth muscle cells via nuclear factor-κB down-modulation'. Together they form a unique fingerprint.

Cite this