Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation

Min Soo Kim, Sangmin Lee, Sanguk Yun, Pann Ghill Suh, Jongmi Park, Minghua Cui, Sun Choi, Sun Shin Cha, Wook Jin

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean ± standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the Pi-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation.

Original languageEnglish
Pages (from-to)1650-1658
Number of pages9
JournalInternational Journal of Biological Macromolecules
StatePublished - Feb 2018

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.


  • DJ-1
  • Inorganic phosphate
  • Parkinson's disease
  • ROS
  • Tartrate


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