@article{73fa6d48a37f41c9a0268dc2c1d063b0,
title = "Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation",
abstract = "The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean ± standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the Pi-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation.",
keywords = "DJ-1, Inorganic phosphate, Parkinson's disease, ROS, Tartrate",
author = "Kim, {Min Soo} and Sangmin Lee and Sanguk Yun and Suh, {Pann Ghill} and Jongmi Park and Minghua Cui and Sun Choi and Cha, {Sun Shin} and Wook Jin",
note = "Funding Information: This research was a part of the project titled “Development of biomedical materials based on marine proteins” funded by the Ministry of Oceans and Fisheries, Korea. This work was also supported by Basic Science Research Program and Global Frontier Research Program through National Research Foundation of Korea (NRF) funded by the korea government (MSIP) (NRF-2015R 1A2A2A 01004168 and NRF-2015M 1A5A 1037480 to S.-S. Cha, NRF-2015M3A6A4065729 to S. Lee, NRF-2017R 1A2B 4010084 to S. C and 2015R1D1A1A01059406 to MS, K), and by the Gachon University Gil Medical Center (2014-14 to W. J.). Funding Information: This research was a part of the project titled “Development of biomedical materials based on marine proteins”, funded by the Ministry of Oceans and Fisheries, Korea . This work was also supported by Basic Science Research Program and Global Frontier Research Program through National Research Foundation of Korea (NRF) funded by the korea government (MSIP) ( NRF-2015R 1A2A2A 01004168 and NRF-2015M 1A5A 1037480 to S.-S. Cha, NRF-2015M3A6A4065729 to S. Lee, NRF-2017R 1A2B 4010084 to S. C and 2015R1D1A1A01059406 to MS, K), and by the Gachon University Gil Medical Center (2014-14 to W. J.). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",
year = "2018",
month = feb,
doi = "10.1016/j.ijbiomac.2017.10.022",
language = "English",
volume = "107",
pages = "1650--1658",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",
}