Inhibition of the benzo[a]pyrene-induced toxicity by allyl sulfides in human epidermal keratinocytes

Hyang Sook Chun; Jung Kim Hyun; Yangha Kim; Joo Chang Hyun

doi:10.1007/s10529-004-3738-2

Inhibition of the benzo[a]pyrene-induced toxicity by allyl sulfides in human epidermal keratinocytes

Hyang Sook Chun, Jung Kim Hyun, Yangha Kim, Joo Chang Hyun

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Diallyl sulfide (DAS) and diallyl disulfide (DADS) at 25 μg ml ^-1 decreased the benzo[a]pyrene (B[a]P)-induced colony growth inhibition of human epidermal keratinocytes. DAS and DADS decreased B[a]P-DNA and B[a]P-protein adducts by 65% and 49-55%, respectively. The B[a]P-induced ethoxyresorufin O-deethylase activity, a marker enzyme for cytochrome P450 1, was decreased from 3 to 1.7-1.9 nmol min^-1 mg^-1 microsomal protein by DAS and DADS treatments. The activity of glutathione S-transferase, a detoxifying enzyme for B[a]P, but was decreased by DADS, but was unaffected by DAS.

Original language	English
Pages (from-to)	1701-1706
Number of pages	6
Journal	Biotechnology Letters
Volume	26
Issue number	22
DOIs	https://doi.org/10.1007/s10529-004-3738-2
State	Published - Nov 2004

Bibliographical note

Funding Information:
This work is supported by research grants from the Korea Institute of Science and Technology Evaluation and Planning (KISTEP) for functional food research and development, Ministry of Science and Technology, in the Republic of Korea.

Keywords

B[a]P-induced toxicity
Diallyl disulfide
Diallyl sulfide
Keratinocyte

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10.1007/s10529-004-3738-2

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title = "Inhibition of the benzo[a]pyrene-induced toxicity by allyl sulfides in human epidermal keratinocytes",

abstract = "Diallyl sulfide (DAS) and diallyl disulfide (DADS) at 25 μg ml -1 decreased the benzo[a]pyrene (B[a]P)-induced colony growth inhibition of human epidermal keratinocytes. DAS and DADS decreased B[a]P-DNA and B[a]P-protein adducts by 65% and 49-55%, respectively. The B[a]P-induced ethoxyresorufin O-deethylase activity, a marker enzyme for cytochrome P450 1, was decreased from 3 to 1.7-1.9 nmol min-1 mg-1 microsomal protein by DAS and DADS treatments. The activity of glutathione S-transferase, a detoxifying enzyme for B[a]P, but was decreased by DADS, but was unaffected by DAS.",

keywords = "B[a]P-induced toxicity, Diallyl disulfide, Diallyl sulfide, Keratinocyte",

author = "Chun, {Hyang Sook} and Hyun, {Jung Kim} and Yangha Kim and Hyun, {Joo Chang}",

note = "Funding Information: This work is supported by research grants from the Korea Institute of Science and Technology Evaluation and Planning (KISTEP) for functional food research and development, Ministry of Science and Technology, in the Republic of Korea.",

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doi = "10.1007/s10529-004-3738-2",

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AU - Chun, Hyang Sook

AU - Hyun, Jung Kim

AU - Kim, Yangha

AU - Hyun, Joo Chang

N1 - Funding Information: This work is supported by research grants from the Korea Institute of Science and Technology Evaluation and Planning (KISTEP) for functional food research and development, Ministry of Science and Technology, in the Republic of Korea.

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N2 - Diallyl sulfide (DAS) and diallyl disulfide (DADS) at 25 μg ml -1 decreased the benzo[a]pyrene (B[a]P)-induced colony growth inhibition of human epidermal keratinocytes. DAS and DADS decreased B[a]P-DNA and B[a]P-protein adducts by 65% and 49-55%, respectively. The B[a]P-induced ethoxyresorufin O-deethylase activity, a marker enzyme for cytochrome P450 1, was decreased from 3 to 1.7-1.9 nmol min-1 mg-1 microsomal protein by DAS and DADS treatments. The activity of glutathione S-transferase, a detoxifying enzyme for B[a]P, but was decreased by DADS, but was unaffected by DAS.

AB - Diallyl sulfide (DAS) and diallyl disulfide (DADS) at 25 μg ml -1 decreased the benzo[a]pyrene (B[a]P)-induced colony growth inhibition of human epidermal keratinocytes. DAS and DADS decreased B[a]P-DNA and B[a]P-protein adducts by 65% and 49-55%, respectively. The B[a]P-induced ethoxyresorufin O-deethylase activity, a marker enzyme for cytochrome P450 1, was decreased from 3 to 1.7-1.9 nmol min-1 mg-1 microsomal protein by DAS and DADS treatments. The activity of glutathione S-transferase, a detoxifying enzyme for B[a]P, but was decreased by DADS, but was unaffected by DAS.

KW - B[a]P-induced toxicity

KW - Diallyl disulfide

KW - Diallyl sulfide

KW - Keratinocyte

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 22

ER -

Inhibition of the benzo[a]pyrene-induced toxicity by allyl sulfides in human epidermal keratinocytes

Abstract

Bibliographical note

Keywords

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