Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

So Young Kim; Eun Jung Lee; Moon Sook Woo; Ji Sun Jung; Jin Won Hyun; Sung Won Min; Dong Hyun Kim; Hee Sun Kim

doi:10.1016/j.bbrc.2007.11.178

Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

So Young Kim
, Eun Jung Lee
, Moon Sook Woo
, Ji Sun Jung
, Jin Won Hyun
, Sung Won Min
, Dong Hyun Kim
, Hee Sun Kim

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

Original language	English
Pages (from-to)	493-499
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	366
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.11.178
State	Published - 8 Feb 2008

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.

Keywords

AP-1
Glioma
Invasion
Irisolidone
MMP-9
NF-κB
Signaling pathways

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10.1016/j.bbrc.2007.11.178

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title = "Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells",

abstract = "Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.",

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note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.",

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AU - Kim, So Young

AU - Lee, Eun Jung

AU - Woo, Moon Sook

AU - Jung, Ji Sun

AU - Hyun, Jin Won

AU - Min, Sung Won

AU - Kim, Dong Hyun

AU - Kim, Hee Sun

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.

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N2 - Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

AB - Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

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KW - NF-κB

KW - Signaling pathways

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Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

Abstract

Bibliographical note

Keywords

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