Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

So Young Kim, Eun Jung Lee, Moon Sook Woo, Ji Sun Jung, Jin Won Hyun, Sung Won Min, Dong Hyun Kim, Hee Sun Kim

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

Original languageEnglish
Pages (from-to)493-499
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number2
DOIs
StatePublished - 8 Feb 2008

Keywords

  • AP-1
  • Glioma
  • Invasion
  • Irisolidone
  • MMP-9
  • NF-κB
  • Signaling pathways

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