Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

So Young Kim; Eun Jung Lee; Moon Sook Woo; Ji Sun Jung; Jin Won Hyun; Sung Won Min; Dong Hyun Kim; Hee Sun Kim

doi:10.1016/j.bbrc.2007.11.178

Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

So Young Kim, Eun Jung Lee, Moon Sook Woo, Ji Sun Jung, Jin Won Hyun, Sung Won Min, Dong Hyun Kim, Hee Sun Kim

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

Original language	English
Pages (from-to)	493-499
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	366
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.11.178
State	Published - 8 Feb 2008

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.

Keywords

AP-1
Glioma
Invasion
Irisolidone
MMP-9
NF-κB
Signaling pathways

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10.1016/j.bbrc.2007.11.178

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title = "Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells",

abstract = "Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.",

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author = "Kim, {So Young} and Lee, {Eun Jung} and Woo, {Moon Sook} and Jung, {Ji Sun} and Hyun, {Jin Won} and Min, {Sung Won} and Kim, {Dong Hyun} and Kim, {Hee Sun}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.",

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AU - Jung, Ji Sun

AU - Hyun, Jin Won

AU - Min, Sung Won

AU - Kim, Dong Hyun

AU - Kim, Hee Sun

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (2006-KFR-531-E00060), and in part by Neurobiology Research Program (M10412000042-04N1200 -04210) of the Ministry of Science and Technology, Republic of Korea.

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N2 - Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

AB - Matrix metalloproteinase-9 (MMP-9) plays an important role in mediating the invasion and angiogenic process of malignant gliomas. This study was undertaken to determine if an isoflavone metabolite, irisolidone, inhibits MMP-9 expression in human astroglioma cells. Irisolidone was found to inhibit the secretion and protein expression of MMP-9 induced by PMA in U87 MG glioma cells, accompanied by the inhibition of MMP-9 mRNA expression and promoter activity. Further mechanistic studies revealed that irisolidone inhibits the binding of NF-κB and AP-1 to the MMP-9 promoter and suppresses the PMA-induced phosphorylation of ERK and JNK, which are upstream signaling molecules in MMP-9 expression. The Matrigel-invasion assay showed that irisolidone suppresses the in vitro invasiveness of glioma cells. Therefore, the strong inhibition of MMP-9 expression by irisolidone might be a potential therapeutic modality for controlling the growth and invasiveness of gliomas.

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Inhibition of matrix metalloproteinase-9 gene expression by an isoflavone metabolite, irisolidone in U87MG human astroglioma cells

Abstract

Bibliographical note

Keywords

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