Influence of subinhibitory concentrations of NH125 on biofilm formation & virulence factors of Staphylococcus aureus

Qingzhong Liu, Zhaojun Zheng, Wooseong Kim, Beth Burgwyn Fuchs, Eleftherios Mylonakis

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Aim: L-benzyl-3-cetyl-2-methylimidazolium iodide (NH125) can inhibit Staphylococcus aureus growth. We investigated the effects of sub-MIC concentrations of NH125 on S. aureus biofilm and virulence. Methodology & results: Three strains of S. aureus were tested. Sub-lethal concentrations of NH125 repressed biofilm formation. At partial sub-MICs, NH125 downregulated the expression of most virulence, while strain-dependent effects were found in the production of α-hemolysin, δ-hemolysin, coagulase and nuclease. In Galleria mellonella model, methicillin-resistant S. aureus pre-exposed to NH125 demonstrated significantly lower killing (p = 0.032 for 1/16 and 1/8 MICs; 0.008 for 1/4 MIC; and 0.001 for 1/2 MIC). Conclusion: Sub-MIC concentrations of NH125 inhibited biofilm formation and virulence of S. aureus. These findings provide further support for evaluating the clinical efficacy of NH125 in staphylococcal infection.

Original languageEnglish
Pages (from-to)1319-1331
Number of pages13
JournalFuture Medicinal Chemistry
Issue number11
StatePublished - Jun 2018

Bibliographical note

Funding Information:
This study was supported by NIH grant P01 AI083214 to E Mylonakis. Q Liu is supported by Shanghai General Hospital Characteristic Discipline Construction Fund and Shanghai Jiao Tong University KC Wong Medical Fellowship Fund. Z Zheng is supported by the China Scholarship Council (CSC) through Chinese Government Graduate Student Overseas Study Program. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Publisher Copyright:
© 2018 Newlands Press.


  • NH125
  • Staphylococcus aureus
  • sub-MIC
  • virulence factor


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