Influence of subinhibitory concentrations of NH125 on biofilm formation & virulence factors of Staphylococcus aureus

Qingzhong Liu, Zhaojun Zheng, Wooseong Kim, Beth Burgwyn Fuchs, Eleftherios Mylonakis

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Aim: L-benzyl-3-cetyl-2-methylimidazolium iodide (NH125) can inhibit Staphylococcus aureus growth. We investigated the effects of sub-MIC concentrations of NH125 on S. aureus biofilm and virulence. Methodology & results: Three strains of S. aureus were tested. Sub-lethal concentrations of NH125 repressed biofilm formation. At partial sub-MICs, NH125 downregulated the expression of most virulence, while strain-dependent effects were found in the production of α-hemolysin, δ-hemolysin, coagulase and nuclease. In Galleria mellonella model, methicillin-resistant S. aureus pre-exposed to NH125 demonstrated significantly lower killing (p = 0.032 for 1/16 and 1/8 MICs; 0.008 for 1/4 MIC; and 0.001 for 1/2 MIC). Conclusion: Sub-MIC concentrations of NH125 inhibited biofilm formation and virulence of S. aureus. These findings provide further support for evaluating the clinical efficacy of NH125 in staphylococcal infection.

Original languageEnglish
Pages (from-to)1319-1331
Number of pages13
JournalFuture Medicinal Chemistry
Volume10
Issue number11
DOIs
StatePublished - Jun 2018

Keywords

  • NH125
  • Staphylococcus aureus
  • sub-MIC
  • virulence factor

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