TY - JOUR
T1 - Influence of matrix rigidity on the internal twisting of electronically excited thioflavin T in polymer nanostructures
AU - Lee, Youmin
AU - Lim, Yu
AU - Hwa Kim, Myung
AU - Lee, Minyung
N1 - Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2A10004510 ).
PY - 2013/11/19
Y1 - 2013/11/19
N2 - Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.
AB - Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.
UR - http://www.scopus.com/inward/record.url?scp=84893770090&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2013.10.002
DO - 10.1016/j.cplett.2013.10.002
M3 - Article
AN - SCOPUS:84893770090
SN - 0009-2614
VL - 588
SP - 141
EP - 144
JO - Chemical Physics Letters
JF - Chemical Physics Letters
ER -