Influence of matrix rigidity on the internal twisting of electronically excited thioflavin T in polymer nanostructures

Youmin Lee; Yu Lim; Myung Hwa Kim; Minyung Lee

doi:10.1016/j.cplett.2013.10.002

Influence of matrix rigidity on the internal twisting of electronically excited thioflavin T in polymer nanostructures

Youmin Lee, Yu Lim, Myung Hwa Kim, Minyung Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Chemical Physics Letters
Volume	588
DOIs	https://doi.org/10.1016/j.cplett.2013.10.002
State	Published - 19 Nov 2013

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2A10004510 ).

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10.1016/j.cplett.2013.10.002

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title = "Influence of matrix rigidity on the internal twisting of electronically excited thioflavin T in polymer nanostructures",

abstract = "Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.",

author = "Youmin Lee and Yu Lim and {Hwa Kim}, Myung and Minyung Lee",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2A10004510 ).",

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doi = "10.1016/j.cplett.2013.10.002",

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AU - Lee, Youmin

AU - Lim, Yu

AU - Hwa Kim, Myung

AU - Lee, Minyung

N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2A10004510 ).

PY - 2013/11/19

Y1 - 2013/11/19

N2 - Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.

AB - Thioflavin T (ThT) exhibits an enormous increase in fluorescence intensity (or lifetime) upon binding to bsheet rich protein aggregates. In this letter, we measured the fluorescence lifetimes of ThT in poly (ethylene oxide) (PEO) and poly (acrylic acid) (PAA) polymer films and nanofibers and observed that the excited-state motion of ThT is slowed down as the mechanical rigidity of medium increases. Using ThT as a rigidity sensor, we first demonstrate that the fluorescence lifetime imaging microscopy (FLIM) can easily distinguish the PEO and PAA nanofiber structures, which were not discernable by electron microscopy.

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DO - 10.1016/j.cplett.2013.10.002

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VL - 588

SP - 141

EP - 144

JO - Chemical Physics Letters

JF - Chemical Physics Letters

ER -

Influence of matrix rigidity on the internal twisting of electronically excited thioflavin T in polymer nanostructures

Abstract

Bibliographical note

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