Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages

Su Jae Lee; Chul Koo Cho; Seong Yul Yoo; Tae Hwan Kim; Yun Sil Lee

doi:10.1016/S0027-5107(99)00078-0

Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages

Su Jae Lee, Chul Koo Cho, Seong Yul Yoo, Tae Hwan Kim, Yun Sil Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Although protein kinase C (PKC) plays an important role in cellular response to radiation, little is known about the specific role of each isoform in the radiation induced cellular response. In this study, the induction of apoptosis and subcellular distribution of PKC isoforms after γ-ray irradiation were examined in three kinds of mouse epidermal cells with different stages of carcinogenesis (normal mouse keratinocytes, PK: v-ras(Ha) transfected mouse keratinocytes, ras-PK; and neoplastic cells from mouse skin papilloma, 308 cells). The induction of apoptosis was different in normal and neoplastic cells; in normal cells after 16 Gy of radiation, apoptosis was 2-10 times higher than that in ras-PK or 308 cells, and was rapidly induced; other cells died more slowly, depending on the stage of carcinogenesis. The responses of each PKC, especially rapid translocation of PKCδ and no response of PKCε by radiation in normal cells may influence the induction of apoptosis by radiation. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	426
Issue number	1
DOIs	https://doi.org/10.1016/S0027-5107(99)00078-0
State	Published - 3 May 1999

Bibliographical note

Funding Information:
This study was supported by a National Project Grant from the Ministry of Science and Technology. We are grateful to Mr. Kyung-Jung Kim and Miss Sun-Ah Choi for their excellent technical assistance.

Keywords

Apoptotic cell death
Cellular redistribution
Gamma ray
Normal cell
Papilloma cell
Protein kinase C isozymes
v-ras(Ha) transformed cell

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10.1016/S0027-5107(99)00078-0

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Lee, S. J., Cho, C. K., Yoo, S. Y., Kim, T. H., & Lee, Y. S. (1999). Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 426(1), 41-49. https://doi.org/10.1016/S0027-5107(99)00078-0

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title = "Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages",

abstract = "Although protein kinase C (PKC) plays an important role in cellular response to radiation, little is known about the specific role of each isoform in the radiation induced cellular response. In this study, the induction of apoptosis and subcellular distribution of PKC isoforms after γ-ray irradiation were examined in three kinds of mouse epidermal cells with different stages of carcinogenesis (normal mouse keratinocytes, PK: v-ras(Ha) transfected mouse keratinocytes, ras-PK; and neoplastic cells from mouse skin papilloma, 308 cells). The induction of apoptosis was different in normal and neoplastic cells; in normal cells after 16 Gy of radiation, apoptosis was 2-10 times higher than that in ras-PK or 308 cells, and was rapidly induced; other cells died more slowly, depending on the stage of carcinogenesis. The responses of each PKC, especially rapid translocation of PKCδ and no response of PKCε by radiation in normal cells may influence the induction of apoptosis by radiation. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Apoptotic cell death, Cellular redistribution, Gamma ray, Normal cell, Papilloma cell, Protein kinase C isozymes, v-ras(Ha) transformed cell",

author = "Lee, {Su Jae} and Cho, {Chul Koo} and Yoo, {Seong Yul} and Kim, {Tae Hwan} and Lee, {Yun Sil}",

note = "Funding Information: This study was supported by a National Project Grant from the Ministry of Science and Technology. We are grateful to Mr. Kyung-Jung Kim and Miss Sun-Ah Choi for their excellent technical assistance.",

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doi = "10.1016/S0027-5107(99)00078-0",

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Lee, SJ, Cho, CK, Yoo, SY, Kim, TH & Lee, YS 1999, 'Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 426, no. 1, pp. 41-49. https://doi.org/10.1016/S0027-5107(99)00078-0

Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages. / Lee, Su Jae; Cho, Chul Koo; Yoo, Seong Yul et al.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 426, No. 1, 03.05.1999, p. 41-49.

Research output: Contribution to journal › Article › peer-review

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Influence of ionizing radiation on induction of apoptotic cell death and cellular redistribution of protein kinase C isozymes in mouse epidermal cells differing in carcinogenesis stages

Abstract

Bibliographical note

Keywords

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