Influence of acid mine drainage on microbial communities in stream and groundwater samples at guryong mine, South korea

Jaisoo Kim; So Yeon Koo; Ji Young Kim; Eun Hee Lee; Sang Don Lee; Kyung Seok Ko; Dong Chan Ko; Kyung Suk Cho

doi:10.1007/s00254-008-1663-8

Influence of acid mine drainage on microbial communities in stream and groundwater samples at guryong mine, South korea

Jaisoo Kim, So Yeon Koo, Ji Young Kim, Eun Hee Lee, Sang Don Lee, Kyung Seok Ko, Dong Chan Ko, Kyung Suk Cho

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1, GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD.

Original language	English
Pages (from-to)	1567-1574
Number of pages	8
Journal	Environmental Geology
Volume	58
Issue number	7
DOIs	https://doi.org/10.1007/s00254-008-1663-8
State	Published - Oct 2009

Bibliographical note

Funding Information:
Acknowledgments This research was supported by a grant (project: Application of groundwater ecology to characterization of groundwater systems and contaminant behavior) from the Korea Research Council of Public Science & Technology. S. Y. Koo and J. Y. Kim were financially supported by the Korea Science and Engineering Foundation (KOSEF) through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0). E. H. Lee was financially supported by the KOSEF NRL Program grant funded by the Korea government (MEST) (R0A-2008-000-20044-0).

Keywords

Acid mine drainage
Groundwater
Microbial community structure
Microbial diversity
Stream

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title = "Influence of acid mine drainage on microbial communities in stream and groundwater samples at guryong mine, South korea",

abstract = "The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1, GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD.",

keywords = "Acid mine drainage, Groundwater, Microbial community structure, Microbial diversity, Stream",

author = "Jaisoo Kim and Koo, {So Yeon} and Kim, {Ji Young} and Lee, {Eun Hee} and Lee, {Sang Don} and Ko, {Kyung Seok} and Ko, {Dong Chan} and Cho, {Kyung Suk}",

note = "Funding Information: Acknowledgments This research was supported by a grant (project: Application of groundwater ecology to characterization of groundwater systems and contaminant behavior) from the Korea Research Council of Public Science & Technology. S. Y. Koo and J. Y. Kim were financially supported by the Korea Science and Engineering Foundation (KOSEF) through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0). E. H. Lee was financially supported by the KOSEF NRL Program grant funded by the Korea government (MEST) (R0A-2008-000-20044-0).",

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AU - Lee, Sang Don

AU - Ko, Kyung Seok

AU - Ko, Dong Chan

AU - Cho, Kyung Suk

N1 - Funding Information: Acknowledgments This research was supported by a grant (project: Application of groundwater ecology to characterization of groundwater systems and contaminant behavior) from the Korea Research Council of Public Science & Technology. S. Y. Koo and J. Y. Kim were financially supported by the Korea Science and Engineering Foundation (KOSEF) through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0). E. H. Lee was financially supported by the KOSEF NRL Program grant funded by the Korea government (MEST) (R0A-2008-000-20044-0).

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N2 - The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1, GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD.

AB - The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1, GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD.

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KW - Groundwater

KW - Microbial community structure

KW - Microbial diversity

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JO - Environmental Geology

JF - Environmental Geology

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ER -

Influence of acid mine drainage on microbial communities in stream and groundwater samples at guryong mine, South korea

Abstract

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Keywords

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