Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)

G. Q. Chen, G. Zhang, S. J. Park, S. Y. Lee

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Large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by Aeromonas hydrophila 4AK4 was examined in a 20, 000 1 fermentor. Cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (PHA) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. When cells first grown in a medium containing 50 g glucose 1-1 were further cultivated after the addition of 50 g lauric acid 1-1 under phosphorus limitation, a final cell concentration, PHA concentration and PHA content of 50 g 1-1, 25 g 1-1, and 50 wt%, respectively, were obtained in 46 h, equivalent to PHA productivity of 0.54 g 1-1 h-1. The copolymer produced was found to be a random copolymer, and the 3HHx fraction was 11 mol%.

Original languageEnglish
Pages (from-to)50-55
Number of pages6
JournalApplied Microbiology and Biotechnology
Issue number1-2
StatePublished - 2001

Bibliographical note

Funding Information:
Acknowledgements This work was supported by Procter & Gamble. We thank many researchers at the Jiangmen Biotechnology Institute (Guangdong, China) for their invaluable contribution to this work. We also thank Dr. Phillip R Green (Procter & Gamble, USA) for NMR and DSC analyses of PHA.


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