TY - JOUR
T1 - Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
AU - Chen, G. Q.
AU - Zhang, G.
AU - Park, S. J.
AU - Lee, S. Y.
N1 - Funding Information:
Acknowledgements This work was supported by Procter & Gamble. We thank many researchers at the Jiangmen Biotechnology Institute (Guangdong, China) for their invaluable contribution to this work. We also thank Dr. Phillip R Green (Procter & Gamble, USA) for NMR and DSC analyses of PHA.
PY - 2001
Y1 - 2001
N2 - Large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by Aeromonas hydrophila 4AK4 was examined in a 20, 000 1 fermentor. Cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (PHA) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. When cells first grown in a medium containing 50 g glucose 1-1 were further cultivated after the addition of 50 g lauric acid 1-1 under phosphorus limitation, a final cell concentration, PHA concentration and PHA content of 50 g 1-1, 25 g 1-1, and 50 wt%, respectively, were obtained in 46 h, equivalent to PHA productivity of 0.54 g 1-1 h-1. The copolymer produced was found to be a random copolymer, and the 3HHx fraction was 11 mol%.
AB - Large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by Aeromonas hydrophila 4AK4 was examined in a 20, 000 1 fermentor. Cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (PHA) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. When cells first grown in a medium containing 50 g glucose 1-1 were further cultivated after the addition of 50 g lauric acid 1-1 under phosphorus limitation, a final cell concentration, PHA concentration and PHA content of 50 g 1-1, 25 g 1-1, and 50 wt%, respectively, were obtained in 46 h, equivalent to PHA productivity of 0.54 g 1-1 h-1. The copolymer produced was found to be a random copolymer, and the 3HHx fraction was 11 mol%.
UR - http://www.scopus.com/inward/record.url?scp=0034784749&partnerID=8YFLogxK
U2 - 10.1007/s002530100755
DO - 10.1007/s002530100755
M3 - Article
C2 - 11693933
AN - SCOPUS:0034784749
SN - 0175-7598
VL - 57
SP - 50
EP - 55
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1-2
ER -