Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid

Hee Kyoung Kang; Jung Han Suh; Jung Jin Lee; Sun Hee Yoon; Jin Won Hyun; Seong Won Choi; Jeong Yun Choi; Kyung Ha Ryu; Myung Hee Chung

doi:10.1080/1071576031000103078

Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid

Hee Kyoung Kang, Jung Han Suh, Jung Jin Lee, Sun Hee Yoon, Jin Won Hyun, Seong Won Choi, Jeong Yun Choi, Kyung Ha Ryu, Myung Hee Chung

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The present study was undertaken to examine the effect of L-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥ 10^-4 M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H₂O₂ produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation.

Original language	English
Pages (from-to)	773-779
Number of pages	7
Journal	Free Radical Research
Volume	37
Issue number	7
DOIs	https://doi.org/10.1080/1071576031000103078
State	Published - 1 Jul 2003

Bibliographical note

Funding Information:
This work was supported by grant from the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.

Keywords

Differentiation
Granulocytes
HL-60
HO
L-ascorbic acid

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title = "Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid",

abstract = "The present study was undertaken to examine the effect of L-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥ 10-4 M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H2O2 produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation.",

keywords = "Differentiation, Granulocytes, HL-60, HO, L-ascorbic acid",

author = "Kang, {Hee Kyoung} and Suh, {Jung Han} and Lee, {Jung Jin} and Yoon, {Sun Hee} and Hyun, {Jin Won} and Choi, {Seong Won} and Choi, {Jeong Yun} and Ryu, {Kyung Ha} and Chung, {Myung Hee}",

note = "Funding Information: This work was supported by grant from the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.",

year = "2003",

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doi = "10.1080/1071576031000103078",

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AU - Kang, Hee Kyoung

AU - Suh, Jung Han

AU - Lee, Jung Jin

AU - Yoon, Sun Hee

AU - Hyun, Jin Won

AU - Choi, Seong Won

AU - Choi, Jeong Yun

AU - Ryu, Kyung Ha

AU - Chung, Myung Hee

N1 - Funding Information: This work was supported by grant from the Ministry of Science and Technology of Korea through the National Research Laboratory Program for Free Radicals.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - The present study was undertaken to examine the effect of L-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥ 10-4 M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H2O2 produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation.

AB - The present study was undertaken to examine the effect of L-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥ 10-4 M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H2O2 produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation.

KW - Differentiation

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Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid

Abstract

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Keywords

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