Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation

Hyun Jai Cho, Choon Soo Lee, Yoo Wook Kwon, Jae Seung Paek, Sun Hee Lee, Jin Hur, Eun Ju Lee, Tae Young Roh, In Sun Chu, Sun Hee Leem, Youngsoo Kim, Hyun Jae Kang, Young Bae Park, Hyo Soo Kim

Research output: Contribution to journalArticlepeer-review

196 Scopus citations


The concept of reprogramming of somatic cells has opened a new era in regenerative medicine. Transduction of defined factors has successfully achieved pluripotency. However, during the generation process of induced pluripotent stem (iPS) cells, genetic manipulation of certain factors may cause tumorigenicity, which limits further application. We report that that a single transfer of embryonic stem (ES) cell-derived proteins into primarily cultured adult mouse fibroblasts, rather than repeated transfer or prolonged exposure to materials, can achieve full reprogramming up to the pluripotent state without the forced expression of ectopic transgenes. During the process, gene expression and epigenetic status were converted from somatic to ES-equivalent status. We verified that protein-based reprogramming was neither by the contamination of protein donor ES cell nor by DNA/RNA from donor ES cell. Protein-iPS cells were biologically and functionally very similar to ES cells and differentiated into 3 germ layers in vitro. Furthermore, protein-iPS cells possessed in vivo differentiation (well-differentiated teratoma formation) and development (chimeric mice generation and a tetraploid blastocyst complementation) potentials. Our results provide an alternative and safe strategy for the reprogramming of somatic cells that can be used to facilitate pluripotent stem cell-based cell therapy.

Original languageEnglish
Pages (from-to)386-395
Number of pages10
Issue number3
StatePublished - 22 Jul 2010


Dive into the research topics of 'Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation'. Together they form a unique fingerprint.

Cite this