Induction of cell death by photodynamic therapy with a new synthetic photosensitizer DH-I-180-3 in undifferentiated and differentiated 3T3-L1 cells

Youn Hee Choi, Si Hwan Ko, Jong Kim Se, Won Young Lee, Han Park Jeon, Jae Myun Lee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

To examine the photodynamic therapy (PDT) effect on adipocytes, we investigated whether PDT using DH-I-180-3, a new synthetic lipophilic photosensitizer, induced cell death of both undifferentiated and differentiated 3T3-L1. 3T3-L1 pre-adipocytes were differentiated into adipocytes in the culture medium containing pantothenate, insulin, dexamethasone, isobutylmethylxanthine, and troglitazone. PDT was applied to both undifferentiated and differentiated 3T3-L1. Photosensitizer uptake in fat cells was determined by measuring its mean fluorescence intensity. DH-I-180-3 mediated effectively PDT-induced cell death of both pre-adipocytes and adipocytes. And the photosensitizer was accumulated more rapidly in 3T3-L1 adipocytes, compared with other cancer cell lines. These results demonstrate that PDT is a potent cell death inducer in pre-adipocytes and adipocytes. Thus, PDT with DH-I-180-3 may be applied for a new therapeutic modality for obesity treatment.

Original languageEnglish
Pages (from-to)1059-1064
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number4
DOIs
StatePublished - 2 Dec 2005

Keywords

  • Adipocytes
  • Cell death
  • DH-I-180-3
  • PDT
  • Pre-adipocytes

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