Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Kyoung Ah Kang; Hee Kyoung Lim; Seung Uk Kim; Yeong Wan Kim; Won Taek Kim; Ha Sook Chung; Min Kyung Choo; Dong Hyun Kim; Hee Sun Kim; Mi Ja Shim; Myung Hee Chung; Jin Won Hyun

doi:10.1111/j.1745-4514.2005.00001.x

Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Kyoung Ah Kang
, Hee Kyoung Lim
, Seung Uk Kim
, Yeong Wan Kim
, Won Taek Kim
, Ha Sook Chung
, Min Kyung Choo
, Dong Hyun Kim
, Hee Sun Kim
, Mi Ja Shim
, Myung Hee Chung
, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

Original language	English
Pages (from-to)	27-40
Number of pages	14
Journal	Journal of Food Biochemistry
Volume	29
Issue number	1
DOIs	https://doi.org/10.1111/j.1745-4514.2005.00001.x
State	Published - Feb 2005

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10.1111/j.1745-4514.2005.00001.x

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@article{cb056ade2d9a44cfb3cece547a085d1e,

title = "Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells",

abstract = "We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.",

author = "Kang, \{Kyoung Ah\} and Lim, \{Hee Kyoung\} and Kim, \{Seung Uk\} and Kim, \{Yeong Wan\} and Kim, \{Won Taek\} and Chung, \{Ha Sook\} and Choo, \{Min Kyung\} and Kim, \{Dong Hyun\} and Kim, \{Hee Sun\} and Shim, \{Mi Ja\} and Chung, \{Myung Hee\} and Hyun, \{Jin Won\}",

year = "2005",

month = feb,

doi = "10.1111/j.1745-4514.2005.00001.x",

language = "English",

volume = "29",

pages = "27--40",

journal = "Journal of Food Biochemistry",

issn = "0145-8884",

publisher = "John Wiley and Sons Inc.",

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}

TY - JOUR

T1 - Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

AU - Kang, Kyoung Ah

AU - Lim, Hee Kyoung

AU - Kim, Seung Uk

AU - Kim, Yeong Wan

AU - Kim, Won Taek

AU - Chung, Ha Sook

AU - Choo, Min Kyung

AU - Kim, Dong Hyun

AU - Kim, Hee Sun

AU - Shim, Mi Ja

AU - Chung, Myung Hee

AU - Hyun, Jin Won

PY - 2005/2

Y1 - 2005/2

N2 - We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

AB - We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

UR - https://www.scopus.com/pages/publications/21144448880

U2 - 10.1111/j.1745-4514.2005.00001.x

DO - 10.1111/j.1745-4514.2005.00001.x

M3 - Article

AN - SCOPUS:21144448880

SN - 0145-8884

VL - 29

SP - 27

EP - 40

JO - Journal of Food Biochemistry

JF - Journal of Food Biochemistry

IS - 1

ER -

Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Abstract

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