Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Kyoung Ah Kang; Hee Kyoung Lim; Seung Uk Kim; Yeong Wan Kim; Won Taek Kim; Ha Sook Chung; Min Kyung Choo; Dong Hyun Kim; Hee Sun Kim; Mi Ja Shim; Myung Hee Chung; Jin Won Hyun

doi:10.1111/j.1745-4514.2005.00001.x

Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Kyoung Ah Kang, Hee Kyoung Lim, Seung Uk Kim, Yeong Wan Kim, Won Taek Kim, Ha Sook Chung, Min Kyung Choo, Dong Hyun Kim, Hee Sun Kim, Mi Ja Shim, Myung Hee Chung, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

Original language	English
Pages (from-to)	27-40
Number of pages	14
Journal	Journal of Food Biochemistry
Volume	29
Issue number	1
DOIs	https://doi.org/10.1111/j.1745-4514.2005.00001.x
State	Published - Feb 2005

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title = "Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells",

abstract = "We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.",

author = "Kang, {Kyoung Ah} and Lim, {Hee Kyoung} and Kim, {Seung Uk} and Kim, {Yeong Wan} and Kim, {Won Taek} and Chung, {Ha Sook} and Choo, {Min Kyung} and Kim, {Dong Hyun} and Kim, {Hee Sun} and Shim, {Mi Ja} and Chung, {Myung Hee} and Hyun, {Jin Won}",

year = "2005",

month = feb,

doi = "10.1111/j.1745-4514.2005.00001.x",

language = "English",

volume = "29",

pages = "27--40",

journal = "Journal of Food Biochemistry",

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TY - JOUR

T1 - Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

AU - Kang, Kyoung Ah

AU - Lim, Hee Kyoung

AU - Kim, Seung Uk

AU - Kim, Yeong Wan

AU - Kim, Won Taek

AU - Chung, Ha Sook

AU - Choo, Min Kyung

AU - Kim, Dong Hyun

AU - Kim, Hee Sun

AU - Shim, Mi Ja

AU - Chung, Myung Hee

AU - Hyun, Jin Won

PY - 2005/2

Y1 - 2005/2

N2 - We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

AB - We report the cytotoxicity of the ginseng saponin metabolite, Compound K (20-0-D-glucopyranosyl-20(S)-protopanaxadiol, 1H901) on various human leukemia cell lines. Compound K had the most effect on U937, a human monocytic leukemia cell line among the tested cell lines. Compound K-treated U937 cells showed characteristics of apoptosis: an exposure of phosphatidylserine from the inner cell membrane to the outer cell membrane, the formation of apoptotic bodies and DNA fragmentation. Compound K induced apoptosis by up-regulating Box, disrupting the mitochondrial membrane potential, and by activating caspase 9 and caspase 3. Therefore, we suggest that Compound K inhibits U937 cell growth by inducing apoptosis through the up-regulation of Bax and caspase activation.

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U2 - 10.1111/j.1745-4514.2005.00001.x

DO - 10.1111/j.1745-4514.2005.00001.x

M3 - Article

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SN - 0145-8884

VL - 29

SP - 27

EP - 40

JO - Journal of Food Biochemistry

JF - Journal of Food Biochemistry

IS - 1

ER -

Induction of apoptosis by ginseng saponin metabolite in U937 human monocytic leukemia cells

Abstract

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