Inducing cellular alignment on micropost arrays via biophysical spatial stimuli

Ryan D. Sochol, Sheng Chih Chang, Adrienne T. Higa, Megan E. Dueck, Luke P. Lee, Song Li, Liwei Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Micropost arrays are widely used to quantify cellular traction forces on the substrate; however, the ability to control cell morphology on micropost arrays has remained a challenge. Here we present a biophysical method for inducing cellular alignment on micropost arrays. Experimental results for bovine aortic endothelial cells (BAECs) revealed that comparatively higher interpost spacing along one axis significantly promoted cellular alignment along the perpendicular axis. By the end of 18-hour studies, BAECs were aligned within 26°±4° (n = 39 cells) of the designated axis on average, with 36% of seeded BAECs aligned within 10° of the designated axis. Thus, this methodology could be readily employed to simultaneously achieve both cellular alignment and traction force quantification.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages813-815
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Biophysics
  • Cell alignment
  • Micropillars
  • Microposts

Fingerprint

Dive into the research topics of 'Inducing cellular alignment on micropost arrays via biophysical spatial stimuli'. Together they form a unique fingerprint.

Cite this