Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction

Tae Jin Song, Il Kwon, Honglim Piao, Jee Eun Lee, Kyeo Rye Han, Yoonkyung Chang, Hyung Jung Oh, Hyun Jung Choi, Kyung Yul Lee, Yong Jae Kim, Ki Hwan Han, Ji Hoe Heo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (CRF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood clot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2±1.1 sec vs. 17.8±1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2±201.1 μm vs. 114.0±145.1 μm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster clot initiation (clotting time, 59.0±7.3 sec vs. 72.8±5.0 sec, p=0.032) and increased clot growth kinetics (α angle, 84.8±0.2° vs. 82.0±0.6°, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2±0.2% vs. 44.6±1.2%, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.

Original languageEnglish
Pages (from-to)754-759
Number of pages6
JournalYonsei Medical Journal
Issue number6
StatePublished - Aug 2018

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Healthcare Technology Research and Development Project, funded by the Ministry for Health and Welfare, Republic of Korea (HI15C2814, HI15C1056), and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01057934 and 2018R1D1A1B07040959 to TJS).

Publisher Copyright:
© Yonsei University College of Medicine 2018.


  • Chronic renal failure
  • Ferric chloride
  • Thromboelastometry
  • Thrombosis


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