TY - JOUR
T1 - Increased production of ω-hydroxynonanoic acid and α,ω-nonanedioic acid from olive oil by a constructed biocatalytic system
AU - Kang, Su Hwan
AU - Kim, Tae Hun
AU - Park, Jin Byung
AU - Oh, Deok Kun
N1 - Funding Information:
This study was funded by the Mid-Career Researcher Program through the National Research Foundation grant funded by the Ministry of Science and ICT, Republic of Korea (No. 2019R1A2C3002781).
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/9/2
Y1 - 2020/9/2
N2 - ω-Hydroxynonanoic acid and α,ω-nonanedioic acid are used for synthesizing diverse chemicals. Although biological methods are developed, their concentrations are low due to the toxicity of high concentrations of the hydrophobic chemicals toward biocatalysts. Here, we constructed a biocatalytic system with high productivity by adding an adsorbent resin and a strong base anion-exchange resin, reducing the solubility of ω-hydroxynonanoic acid and α,ω-nonanedioic acid, feeding ω-hydroxynonanoic acid, and introducing a cofactor regeneration system. The constructed biocatalytic system converted 300 mM (83.9 g L-1) and 154 mM (43.5 g L-1) oleic acid in the olive oil hydrolysate obtained after resin extraction, which were derived from 110 and 54 g L-1 olive oil, respectively, into 202 mM (35.2 g L-1) ω-hydroxynonanoic acid and 103 mM (19.4 g L-1) α,ω-nonanedioic acid, which are 21- and 24-fold higher values than the previously reported results, respectively. This study may contribute to the industrial biosynthesis of ω-hydroxynonanoic acid and α,ω-nonanedioic acid from olive oil.
AB - ω-Hydroxynonanoic acid and α,ω-nonanedioic acid are used for synthesizing diverse chemicals. Although biological methods are developed, their concentrations are low due to the toxicity of high concentrations of the hydrophobic chemicals toward biocatalysts. Here, we constructed a biocatalytic system with high productivity by adding an adsorbent resin and a strong base anion-exchange resin, reducing the solubility of ω-hydroxynonanoic acid and α,ω-nonanedioic acid, feeding ω-hydroxynonanoic acid, and introducing a cofactor regeneration system. The constructed biocatalytic system converted 300 mM (83.9 g L-1) and 154 mM (43.5 g L-1) oleic acid in the olive oil hydrolysate obtained after resin extraction, which were derived from 110 and 54 g L-1 olive oil, respectively, into 202 mM (35.2 g L-1) ω-hydroxynonanoic acid and 103 mM (19.4 g L-1) α,ω-nonanedioic acid, which are 21- and 24-fold higher values than the previously reported results, respectively. This study may contribute to the industrial biosynthesis of ω-hydroxynonanoic acid and α,ω-nonanedioic acid from olive oil.
KW - biotransformation
KW - olive oil
KW - resin binding
KW - solubility decrease
KW - substrate feeding
KW - α,ω-nonanedioic acid
KW - ω-hydroxynonanoic acid
UR - http://www.scopus.com/inward/record.url?scp=85090249763&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.0c03255
DO - 10.1021/acs.jafc.0c03255
M3 - Article
C2 - 32786834
AN - SCOPUS:85090249763
VL - 68
SP - 9488
EP - 9495
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 35
ER -