Increased Expression of Ornithine Decarboxylase by Gamma-ray in Mouse Epidermal Cells: Relationship with Protein Kinase C Signaling Pathway

Hyun Jung Song, Tai Hwan Kim, Chul Koo Cho, Seong Yul Yoo, Kyung Sook Park, Yun Sil Lee

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The correlation between ornithine decarboxylase (ODC) protein induction and specific protein kinase C (PKC) isozyme expression by gamma-ray in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated normal and v-rasHa transformed mouse keratinocytes was examined. TPA at 100 nM was treated in primary mouse keratinocytes immediately after 4 Gy, 8 Gy and 16 Gy gamma-ray irradiation. After 4 hrs, cells were harvested and the protein expression levels of PKC isozymes (PKCα, -δ, -ε, -η and -ζ) and ODC were examined. For v-rasHa infection, primary keratinocytes were infected with a defected retrovirus containing the v-rasHa gene. After 3 hrs of irradiation, each PKC isozyme and ODC protein expression were tested. Gamma-ray increases ODC protein expression in both TPA-treated normal and v-rasHa transformed mouse keratinocytes and this phenomenon correlated to the increased induction of PKCα without altering other PKC isozymes. Tyrosine phosphorylation of epidermal growth factor receptor protein was also stimulated during gamma-ray induced cellular changes in TPA-treated normal mouse keratinocytes. These results indicate that PKCα as an important regulator of mouse epidermal changes by gamma-radiation, contributes to the ODC expression occurring during exposure to tumor promoter, such as TPA, and epidermal neoplasia induced by ras activation.

Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalJournal of Radiation Research
Volume39
Issue number3
DOIs
StatePublished - Sep 1998

Keywords

  • Gamma-ray
  • Mouse epidermal cell
  • Ornithine decarboxylase
  • Protein kinase c isozymes
  • V-ras

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