Effects of oxidized low-density lipoprotein (ox-LDL), l-α-stearoyl-lysophosphatidylcholine (LPC), on intracellular Ca2+ concentration were examined in mouse endothelial cells by measuring intracellular Ca2+ concentration ([Ca2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2+]i under the condition of 1.5 mM [Ca2+]o but did not show any effect under the nominally Ca2+-free condition. Even after the store depletion with 30 μM 2,5-di-tert-butylhydroquinone (BHQ) or 30 μM ATP, LPC could still increase the [Ca2+]i under the condition of 1.5 mM [Ca2+]o. The time required to increase [Ca2+]i (about 1 minute) was longer than that for ATP-induced [Ca2+]i increase (10 ∼ 30 seconds). LPC-induced [Ca2+]i increase was completely blocked by 1 μM La3+. Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased [Ca2+]i via the increase of Ca2+ influx through the Ca2+ routes which exist in the plasma membrane.
|Number of pages||7|
|Journal||Korean Journal of Physiology and Pharmacology|
|State||Published - 2002|
- 2.5-di-tertbutylhydroquinone (BHQ)
- Endothelial cell (EC)
- Lysophosphatidylcholine (LPC)
- Oxidized low-density lipoprotein (ox-LDL)