@article{8f3ec762b8a04ad3ac60e28b25219b78,
title = "Increase in furfural tolerance by combinatorial overexpression of NAD salvage pathway enzymes in engineered isobutanol-producing E. coli",
abstract = "To reduce the furfural toxicity for biochemical production in E. coli, a new strategy was successfully applied by supplying NAD(P)H through the nicotine amide salvage pathway. To alleviate the toxicity, nicotinamide salvage pathway genes were overexpressed in recombinant, isobutanol-producing E. coli. Gene expression of pncB and nadE respectively showed increased tolerance to furfural among these pathways. The combined expression of pncB and nadE was the most effective in increasing the tolerance of the cells to toxic aldehydes. By comparing noxE- and fdh-harbouring strains, the form of NADH, rather than NAD+, was the major effector of furfural tolerance. Overall, this study is the application of the salvage pathway to isobutanol production in the presence of furfural, and this system seems to be applicable to alleviate furfural toxicity in the production of other biochemical.",
keywords = "E. coli, Furfural, Isobutanol, NADH, nadE, pncB",
author = "Song, {Hun Suk} and Jeon, {Jong Min} and Kim, {Hyun Joong} and Bhatia, {Shashi Kant} and Ganesan Sathiyanarayanan and Junyoung Kim and {Won Hong}, Ju and {Gi Hong}, Yoon and {Young Choi}, Kwon and Kim, {Yun Gon} and Wooseong Kim and Yang, {Yung Hun}",
note = "Funding Information: The study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education ( NRF-2015R1A2A2A04006014 , NRF-2016R1D1A1B03932301 , NRF-2015M1A5A1037196 ). Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) is kindly appreciated. Funding Information: The study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2015R1A2A2A04006014, NRF-2016R1D1A1B03932301, NRF-2015M1A5A1037196). Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) is kindly appreciated. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",
year = "2017",
month = dec,
doi = "10.1016/j.biortech.2017.05.197",
language = "English",
volume = "245",
pages = "1430--1435",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Ltd.",
}