Increase in furfural tolerance by combinatorial overexpression of NAD salvage pathway enzymes in engineered isobutanol-producing E. coli

Hun Suk Song, Jong Min Jeon, Hyun Joong Kim, Shashi Kant Bhatia, Ganesan Sathiyanarayanan, Junyoung Kim, Ju Won Hong, Yoon Gi Hong, Kwon Young Choi, Yun Gon Kim, Wooseong Kim, Yung Hun Yang

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

To reduce the furfural toxicity for biochemical production in E. coli, a new strategy was successfully applied by supplying NAD(P)H through the nicotine amide salvage pathway. To alleviate the toxicity, nicotinamide salvage pathway genes were overexpressed in recombinant, isobutanol-producing E. coli. Gene expression of pncB and nadE respectively showed increased tolerance to furfural among these pathways. The combined expression of pncB and nadE was the most effective in increasing the tolerance of the cells to toxic aldehydes. By comparing noxE- and fdh-harbouring strains, the form of NADH, rather than NAD+, was the major effector of furfural tolerance. Overall, this study is the application of the salvage pathway to isobutanol production in the presence of furfural, and this system seems to be applicable to alleviate furfural toxicity in the production of other biochemical.

Original languageEnglish
Pages (from-to)1430-1435
Number of pages6
JournalBioresource Technology
Volume245
DOIs
StatePublished - Dec 2017

Keywords

  • E. coli
  • Furfural
  • Isobutanol
  • NADH
  • nadE
  • pncB

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