Incorporation of quantum dots on virus in polycationic solution

Jin Oh You, Yu San Liu, Yu Chuan Liu, Kye Il Joo, Ching An Peng

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Developing methods to label viruses with fluorescent moieties has its merits in elucidating viral infection mechanisms and exploring novel antiviral therapeutics. Fluorescent quantum dots (QDs), an emerging probe for biological imaging and medical diagnostics, were employed in this study to tag retrovirus encoding enhanced green fluorescent protein (EGFP) genes. Electrostatic repulsion forces generated from both negatively charged retrovirus and QDs were neutralized by cationic Polybrene®, forming colloidal complexes of QDs-virus. By examining the level of EGFP expression in 3T3 fibroblast cells treated with QDs-tagged retroviruses for 24 hours, the infectivity of retrovirus incorporated with QDs was shown to be only slightly decreased. Moreover, the imaging of QDs can be detected in the cellular milieu. In summary, the mild method developed here makes QDs-tagged virus a potential imaging probe for direct tracking the infection process and monitoring distribution of viral particles in infected cells.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalInternational Journal of Nanomedicine
Volume1
Issue number1
DOIs
StatePublished - 2006

Keywords

  • Endocytosis
  • Envelope protein
  • Infection
  • Polybrenespi®
  • Quantum dots
  • Retroviral transduction

Fingerprint

Dive into the research topics of 'Incorporation of quantum dots on virus in polycationic solution'. Together they form a unique fingerprint.

Cite this