In vitro Test for the Osteogenesis of BMSCs with DBP Contents in DBP/PLGA Scaffold

Mi Ock Baek, Soon Hee Kim, Jung Won So, Ji Ye Lim, Hye Won Roh, Na Ri Lee, Gyu Ha Ryu, Yang Ha Cho, Seung Jin Lee, Gilson Khang

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Demineralized bone particles(DBP) exhibit osteoinductive properties and are very useful for bone formation and other orthopeadic applications. In this study, we investigated the osteogenic capabilities of rat bone marrow stromal cells(rBMSCs) in PLGA scaffold impregnated with various DBP contents. DBP-loaded PLGA scaffold (10, 20, 40, 80 % w/w) were prepared by solvent casting/salt leaching method. rBMSCs were seeded in PLGA and DBP/PLGA scaffolds, and then cellular viability and proliferation were assayed by water-soluble tetrazolium salt(WST-8) assay. Osteoblast differentiation of cells were analyzed by alkaline phosphatase(ALP) activity. Finally, reverse transcript polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression of the genes (ALP, osteocalcine and type I collagen) of osteogenic marker in rBMSCs depending on various content of DBP. These results could demonstrated that presence of DBP in PLGA might play important roles in osteoblast differentiation of rBMSCs.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalTissue Engineering and Regenerative Medicine
Issue number1
StatePublished - Mar 2008


  • BMSC
  • DBP
  • DBP/PGLA scaffold
  • Osteogenesis


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