TY - JOUR
T1 - In vitro photodynamic effect of phycocyanin against breast cancer cells
AU - Bharathiraja, Subramaniyan
AU - Seo, Hansu
AU - Manivasagan, Panchanathan
AU - Moorthy, Madhappan Santha
AU - Park, Suhyun
AU - Oh, Jungwan
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - C-phycocyanin, a natural blue-colored pigment-protein complex was explored as a novel photosensitizer for use in low-level laser therapy under 625-nm laser illumination. C-phycocyanin produced singlet oxygen radicals and the level of reactive oxygen species (ROS) were raised in extended time of treatment. It did not exhibit any visible toxic effect in the absence of light. Under 625-nm laser irradiation, c-phycocyanin generated cytotoxic stress through ROS induction, which killed MDA-MB-231 breast cancer cells depending on concentrations. Different fluorescent staining of laser-treated cells explored apoptotic cell death characteristics like the shrinking of cells, cytoplasmic condensation, nuclei cleavage, and the formation of apoptotic bodies. In conclusion, phycocyanin is a non-toxic fluorescent pigment that can be used in low-level light therapy.
AB - C-phycocyanin, a natural blue-colored pigment-protein complex was explored as a novel photosensitizer for use in low-level laser therapy under 625-nm laser illumination. C-phycocyanin produced singlet oxygen radicals and the level of reactive oxygen species (ROS) were raised in extended time of treatment. It did not exhibit any visible toxic effect in the absence of light. Under 625-nm laser irradiation, c-phycocyanin generated cytotoxic stress through ROS induction, which killed MDA-MB-231 breast cancer cells depending on concentrations. Different fluorescent staining of laser-treated cells explored apoptotic cell death characteristics like the shrinking of cells, cytoplasmic condensation, nuclei cleavage, and the formation of apoptotic bodies. In conclusion, phycocyanin is a non-toxic fluorescent pigment that can be used in low-level light therapy.
KW - Laser therapy
KW - Photodynamic therapy
KW - Phycocyanin
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84997633415&partnerID=8YFLogxK
U2 - 10.3390/molecules21111470
DO - 10.3390/molecules21111470
M3 - Article
C2 - 27827890
AN - SCOPUS:84997633415
SN - 1420-3049
VL - 21
JO - Molecules
JF - Molecules
IS - 11
M1 - 1470
ER -