This paper describes a single-aperture MEMS probe and a MEMS probe array for the measurement of biological properties. We designed and fabricated the single-aperture MEMS probe using surface micromachining and verified it by measuring the permittivity of a standard liquid before introducing the MEMS probe array. The actual aperture size of the single-aperture MEMS probe is only 390 νm × 80 νm, which is very small in comparison with the conventional laser-machined coaxial probe. In order to show the feasibility of the proposed single-aperture MEMS probe for permittivity measurements, we performed in vitro measurements of 0.9% saline. Once the single-aperture probe was verified, we proposed the concept of a probe array for biological measurements and experimentally showed the suitability of the MEMS probe array for biological applications through experiments using pork. The MEMS probe array consists of five microstrip feed lines, each of which is followed by open-ended strip lines, and the permittivity measurement of each port is separately performed through the use of a conventional multiport coaxial switch (Agilent, HP 87106 C), followed by a network analyzer (HP 8510 C). Through broadband measurements of 0.9% saline and pork using the MEMS probe array, we were able to discriminate the muscle and fat of pork through just one contact by placing the MEMS probe array on the boundary of muscle and fat. This newly proposed MEMS probe array has great potential in terms of disposability, low cost, integration with planar circuits and a short detection time for biological measurements.