In situ thermal gelling polypeptide for chondrocytes 3D culture

Bo Gyu Choi, Min Hee Park, So Hye Cho, Min Kyung Joo, Hye Jin Oh, Eun Hye Kim, Kwideok Park, Dong Keun Han, Byeongmoon Jeong

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

In the search for a cell-instructive or cell-interactive artificial extracellular matrix, synthetic hydrogels have been extensively investigated to apply three-dimensional (3D) cell culture and tissue engineering. Here, we are reporting a reverse thermal gelling l/. dl-polyalanine block copolymer aqueous solution for chondrocyte 3D culture. The polymer aqueous solution undergoes sol-to-gel transition as the temperature increases, thus forming a 3D cell encapsulating scaffold in situ at 37 °C. In particular, the fraction of the β-sheet structure of the polyalanine dictated the population and thickness of fibrous nanostructure of the hydrogel, which in turn affected the proliferation and protein expression of the encapsulated chondrocytes. As an injectable tissue engineering system of chondrocytes, very promising results were confirmed for nude mice, using the current polypeptide aqueous solution. This paper not only provides important clues in designing an artificial extracellular matrix but also proves the significance of thermal gelling polypeptide as a minimally-invasive tissue engineering scaffold.

Original languageEnglish
Pages (from-to)9266-9272
Number of pages7
JournalBiomaterials
Volume31
Issue number35
DOIs
StatePublished - Dec 2010

Bibliographical note

Funding Information:
This research was supported by the Mid-career Researcher Program (Grant number: 2010-0000832 ), Basic Research Program ( 313-2008-2-C00590 ) and 2010-0001487 through NRF grant funded by the MEST of Korea , and Pioneer Research Center Program through the National Research Foundation of Korea funded by the MEST ( 2010-0002170 ), a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea ( K00060-282 ).

Keywords

  • Cell culture
  • Chondrocyte
  • Hydrogel
  • Peptide
  • Secondary structure
  • Thermally responsive material

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