In-situ food spoilage monitoring using a wireless chemical receptor-conjugated graphene electronic nose

Kyung Ho Kim, Chul Soon Park, Seon Joo Park, Jinyeong Kim, Sung Eun Seo, Jai Eun An, Siyoung Ha, Joonwon Bae, Sooyeol Phyo, Jiwon Lee, Kayoung Kim, Dongseok Moon, Tai Hyun Park, Hyun Seok Song, Oh Seok Kwon

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Monitoring food spoilage is one of the most effective methods for preventing food poisoning caused by biogenic amines or microbes. Therefore, various analytical techniques have been introduced to detect low concentrations of cadaverine (CV) and putrescine (PT), which are representative biogenic polyamines involved in food spoilage (5–8 ppm at the stage of initial decomposition after storage for 5 days at 5 °C and 17–186 ppm at the stage of advanced decomposition after storage for 7 days at 5 °C). Although previous methods showed selective CV and PT detection even at low concentrations, the use of these methods remains challenging in research areas that require in-situ, real-time, on-site monitoring. In this study, we demonstrated for the first time an in-situ high-performance chemical receptor-conjugated graphene electronic nose (CRGE-nose) whose limits of detection (LODs), 27.04 and 7.29 ppb, for CV and PT are up to 102 times more sensitive than those of conventional biogenic amine sensors. Specifically, the novel chemical receptors 2,7-bis(3-morpholinopropyl)benzo[lmn][3,8] phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiMor (NPM)) and 2,7-bis(2-((3-morpholinopropyl)amino)ethyl)benzo[lmn][3,8]phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiEtAmMor (NPEAM)) were designed on the basis of density functional theory (DFT) calculations, and their interaction mechanism was characterized by a DFT 3D simulation. Interestingly, the CRGE-nose was connected on a micro sim chip substrate via wire bonding and then integrated into wireless portable devices, resulting in a cost-effective, high-performance prototype CRGE-nose device capable of on-site detection. The portable CRGE-nose can be used for in-situ monitoring of CV and PT concentration changes as low as 27.04 and 7.29 ppb in real meats such as pork, beef, lamb and chicken.

Original languageEnglish
Article number113908
JournalBiosensors and Bioelectronics
Volume200
DOIs
StatePublished - 15 Mar 2022

Bibliographical note

Funding Information:
This work was supported by the Technology Innovation Program (Project No. 20012362 ) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) ; Smart Farm Innovation Technology Development Program( 421020-03 ); the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT ( NRF-2021M3A9I5021439 ); the Research Program to Solve Urgent Safety Issues of the National Research Foundation of Korea(NRF) funded by the Korean government (Ministry of Science and ICT(MSIT)) ( NRF-2020M3E9A1111636 ); the KIST Institutional Program ( 2E31281 ); Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ014790022021 ) Rural Development Administration; the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program ( 1711134045 ).

Publisher Copyright:
© 2021

Keywords

  • Cadaverine
  • Chemical receptor
  • Gas sensor
  • Graphene
  • Portable biosensors
  • Putrescine
  • Real-time monitoring

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