In-house zinc sad phasing at Cu K? Edge

Min Kyu Kim, Sangmin Lee, Young Jun An, Chang Sook Jeong, Chang Jun Ji, Jin Won Lee, Sun Shin Cha

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

De novo zinc single-wavelength anomalous dispersion (Zn-SAD) phasing has been demonstrated with the 1.9 A resolution data of glucose isomerase and 2.6 A resolution data of Staphylococcus aureus Fur (SaFur) collected using in-house Cu K? X-ray source. The successful in-house Zn-SAD phasing of glucose isomerase, based on the anomalous signals of both zinc ions introduced to crystals by soaking and native sulfur atoms, drove us to determine the structure of SaFur, a zinc-containing transcription factor, by Zn-SAD phasing using in-house X-ray source. The abundance of zinc-containing proteins in nature, the easy zinc derivatization of the protein surface, no need of synchrotron access, and the successful experimental phasing with the modest 2.6 A resolution SAD data indicate that inhouse Zn-SAD phasing can be widely applicable to structure determination.

Original languageEnglish
Pages (from-to)74-81
Number of pages8
JournalMolecules and Cells
Volume36
Issue number1
DOIs
StatePublished - Jul 2013

Keywords

  • Anomalous Scattering
  • Experimental Phasing
  • Protein Crystallography
  • Sad Phasing
  • Zinc

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