In-house zinc sad phasing at Cu K? Edge

Min Kyu Kim, Sangmin Lee, Young Jun An, Chang Sook Jeong, Chang Jun Ji, Jin Won Lee, Sun Shin Cha

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


De novo zinc single-wavelength anomalous dispersion (Zn-SAD) phasing has been demonstrated with the 1.9 A resolution data of glucose isomerase and 2.6 A resolution data of Staphylococcus aureus Fur (SaFur) collected using in-house Cu K? X-ray source. The successful in-house Zn-SAD phasing of glucose isomerase, based on the anomalous signals of both zinc ions introduced to crystals by soaking and native sulfur atoms, drove us to determine the structure of SaFur, a zinc-containing transcription factor, by Zn-SAD phasing using in-house X-ray source. The abundance of zinc-containing proteins in nature, the easy zinc derivatization of the protein surface, no need of synchrotron access, and the successful experimental phasing with the modest 2.6 A resolution SAD data indicate that inhouse Zn-SAD phasing can be widely applicable to structure determination.

Original languageEnglish
Pages (from-to)74-81
Number of pages8
JournalMolecules and Cells
Issue number1
StatePublished - Jul 2013

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant 2012005978, the CAP through Korea Research Council of Fundamental Science Technology (KRCF), Korea Institute of Science and Technology (KIST), & Korea Institute of Ocean Science and Technology (KIOST), the Marine and Extreme Genome Research Center program and the Development of Biohydrogen Production Technology Using Hyperthermophilic Archaea program of MLTM, and the KIOST in-house NSC program. The work performed at Hanyang University was supported by the Korea Research Foundation Grant funded by the Korean Goverment (KRF-2008-313-C00774) and by Mid-career Researcher Program through NRF grant funded by the [The Ministry of Education, Science and Technology (MEST)] (NRF-2012-0005445).


  • Anomalous Scattering
  • Experimental Phasing
  • Protein Crystallography
  • Sad Phasing
  • Zinc


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