Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

Tam Dinh Le Vo; Van Dung Pham; Ji Seun Ko; Seung Hwan Lee; Si Jae Park; Soon Ho Hong

doi:10.1007/s12257-013-0713-6

Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

Tam Dinh Le Vo, Van Dung Pham, Ji Seun Ko, Seung Hwan Lee, Si Jae Park, Soon Ho Hong

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.

Original language	English
Pages (from-to)	327-331
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	19
Issue number	2
DOIs	https://doi.org/10.1007/s12257-013-0713-6
State	Published - Mar 2014

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.

Keywords

Escherichia coli
Pyrococcus horikoshii
gamma-aminobutyric acid
glutamate decarboxylase

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title = "Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli",

abstract = "In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.",

keywords = "Escherichia coli, Pyrococcus horikoshii, gamma-aminobutyric acid, glutamate decarboxylase",

author = "Vo, {Tam Dinh Le} and Pham, {Van Dung} and Ko, {Ji Seun} and Lee, {Seung Hwan} and Park, {Si Jae} and Hong, {Soon Ho}",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.",

year = "2014",

month = mar,

doi = "10.1007/s12257-013-0713-6",

language = "English",

volume = "19",

pages = "327--331",

journal = "Biotechnology and Bioprocess Engineering",

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T1 - Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

AU - Vo, Tam Dinh Le

AU - Pham, Van Dung

AU - Ko, Ji Seun

AU - Lee, Seung Hwan

AU - Park, Si Jae

AU - Hong, Soon Ho

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ00954904), Rural Development Administration, Republic of Korea.

PY - 2014/3

Y1 - 2014/3

N2 - In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.

AB - In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.

KW - Escherichia coli

KW - Pyrococcus horikoshii

KW - gamma-aminobutyric acid

KW - glutamate decarboxylase

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U2 - 10.1007/s12257-013-0713-6

DO - 10.1007/s12257-013-0713-6

M3 - Article

AN - SCOPUS:84901233945

SN - 1226-8372

VL - 19

SP - 327

EP - 331

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 2

ER -

Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

Abstract

Bibliographical note

Keywords

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