Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

Tam Dinh Le Vo, Van Dung Pham, Ji Seun Ko, Seung Hwan Lee, Si Jae Park, Soon Ho Hong

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume19
Issue number2
DOIs
StatePublished - Mar 2014

Keywords

  • Escherichia coli
  • gamma-aminobutyric acid
  • glutamate decarboxylase
  • Pyrococcus horikoshii

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