Improved viability and activity of neutrophils differentiated from HL-60 cells by co-culture with adipose tissue-derived mesenchymal stem cells

Yoon Shin Park, Goh Woon Lim, Kyung Ah Cho, So Youn Woo, Meeyoung Shin, Eun Sun Yoo, Jeong Chan Ra, Kyung Ha Ryu

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Neutropenia is a principal complication of cancer treatment. We investigated the supportive effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) on the viability and function of neutrophils. Neutrophils were derived from HL-60 cells by dimethylformamide stimulation and cultured with or without AD-MSCs under serum-starved conditions to evaluate neutrophil survival, proliferation, and function. Serum starvation resulted in the apoptosis of neutrophils and decreased cell survival. The co-culture of neutrophils and AD-MSCs resulted in cell survival and inhibited neutrophil apoptosis under serum-starved conditions. The survival rate of neutrophils was prolonged up to 72. h, and the expression levels of interferon (IFN)-α, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and transforming growth factor (TGF)-β in AD-MSCs were increased after co-culture with neutrophils. AD-MSCs promoted the viability of neutrophils by inhibiting apoptosis as well as enhancing respiratory burst, which could potentially be mediated by the increased expression of IFN-α, G-CSF, and TGF-β. Thus, we conclude that the use of AD-MSCs may be a promising cell-based therapy for increasing immunity by accelerating neutrophil function.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume423
Issue number1
DOIs
StatePublished - 22 Jun 2012

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2010-0002841).

Keywords

  • Adipose tissue-derived mesenchymal stem cells
  • Differentiated HL-60 cells
  • Neutropenia
  • Neutrophils

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