Improved Productivity of Naringin Oleate with Flavonoid and Fatty Acid by Efficient Enzymatic Esterification

Jonghwa Lee, Kyeonga Kim, Jemin Son, Hyeseon Lee, Jin Han Song, Taek Lee, Heungbae Jeon, Hyun Soo Kim, Si Jae Park, Hah Young Yoo, Chulhwan Park

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Naringin is a flavonoid found in citrus fruits. It exhibits biological activities, such as anticancer and antioxidant effects, but it suffers from low solubility and low stability in lipophilic systems. These drawbacks lead to difficulties in the commercial application of naringin, but they can be overcome through esterification. In this study, naringin oleate was synthesized by enzymatic esterification and optimal conditions for the reaction were investigated. Experiments were conducted focusing on the following parameters: enzyme type, enzyme concentration, molar ratio of naringin to oleic acid, reaction temperature, and reaction solvent. We further confirmed the degree of esterification based on the difference in the initial and the final naringin concentrations. A conversion of 93.10% was obtained under optimized conditions (Lipozyme TL IM 10 g/L, molar ratio 1:20, reaction temperature 40 C, acetonitrile as solvent, and 48 h reaction time). Thus, naringin oleate, a high value-added material that overcomes the low hydrophobicity of naringin and enhances its performance, was obtained through esterification of naringin using oleic acid. This study presented a method for the efficient enzymatic synthesis that could ensure high conversion within a shorter reaction time compared with that required in previously reported methods.

Original languageEnglish
Article number242
Issue number2
StatePublished - Feb 2022


  • Antioxidant
  • Enzymatic synthesis
  • Esterification
  • Flavonoid
  • Flavonoid ester
  • Flavonoid oleate
  • Lipase
  • Naringin
  • Optimization


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