Impaired IL-2 expression in latent HIV-1 infection

Younghyun Shin, Cheol Hee Yoon, Hoyong Lim, Jihwan Park, Tae Young Roh, Chun Kang, Byeong Sun Choi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Regarding the T cell function in HIV-1 infection, activation of T cells is enhanced in acutely HIV-1-infected T cells upon stimuli. However, T cell immune responses underlying the activation of T cell receptor (TCR) signaling molecules and interleukin (IL)-2 production in latently HIV-1-infected cells are poorly understood. The expression and activation of TCR components and its downstream molecules in acutely and latently HIV-1-infected T cells were compared using quantitative reverse transcription polymerase chain reaction (RT-PCR) for mRNA expression and enzyme-linked immunosorbent assay (ELISA) for levels of IL-2 in phytohemagglutinin M (PHA-M). The levels of T cell surface molecules and TCR signaling molecules in latently HIV-1-infected cells were greatly decreased without changes in their mRNA levels. In addition, downstream TCR-signaling molecules in latently HIV-1-infected cells were not activated even in the presence of PHA-M. The phosphorylation of mitogen-activated protein kinases (MAPKs) in the presence of PHA-M was weakly induced in latently HIV-1-infected cells but was greater in acutely HIVNL4-3-infected cells. Finally, the production of IL-2 was significantly decreased in latently HIV-1-infected cells compared with uninfected parent cells. Thus, IL-2-related immunological functions in latently HIV-1-infected T cells were markedly impaired even in the presence of stimuli.

Original languageEnglish
Pages (from-to)1237-1242
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume463
Issue number4
DOIs
StatePublished - 7 Aug 2015

Bibliographical note

Funding Information:
We are grateful to the AIDS Research and Reference Reagent Program (ARRRP, NIH, USA) for providing cell lines (A3.01, ACH-2, Jurkat and J1.1 cells) and HIV-1 NL4-3 strain. We thank to K.C.Kim for their assistance in this project. This work was supported by an intramural grant from the Korea National Institute of Health (Grant Number: 2013-N51001-00 ).

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

  • IL-2
  • Latent HIV-1 infection
  • MAPKs
  • TCR-downstream signaling molecules

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