Impacts of Asian dust events on atmospheric fungal communities

Eun Mi Jeon; Yong Pyo Kim; Kweon Jeong; Ik Soo Kim; Suk Won Eom; Young Zoo Choi; Jong Ok Ka

doi:10.1016/j.atmosenv.2013.08.039

Impacts of Asian dust events on atmospheric fungal communities

Eun Mi Jeon, Yong Pyo Kim, Kweon Jeong, Ik Soo Kim, Suk Won Eom, Young Zoo Choi, Jong Ok Ka

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The composition of atmospheric fungi in Seoul during Asian dust events were assessed by culturing and by molecular methods such as mold specific quantitative PCR (MSQPCR) and internal transcribed spacer cloning (ITS cloning). Culturable fungal concentrations in the air were monitored from May 2008 to July 2011 and 3 pairs of ITS clone libraries, one during Asian dust (AD) day and the other during the adjacent non Asian dust (NAD) day for each pair, were constructed after direct DNA extraction from total suspended particles (TSP) samples. In addition, six aeroallergenic fungi in the atmosphere were also assessed by MSQPCR from October, 2009 to November, 2011. The levels of the airborne culturable fungal concentrations during AD days was significantly higher than that of NAD days (P<0.005). In addition, the correlation of culturable fungal concentrations with particulate matters equal to or less than 10μm in aerodynamic diameter (PM₁₀) concentrations was observed to be high (0.775) for the AD days while correlation coefficients of PM₁₀ as well as other particulate parameters with airborne fungal concentrations were significantly negative for the NAD days during intensive monitoring periods (May to June, 2008). It was found that during AD days several airborne allergenic fungal levels measured with MSQPCR increased up to 5-12 times depending on the species. Comparison of AD vs. NAD clones showed significant differences (P<0.05) in all three cases using libshuff. In addition, high proportions of uncultured soil fungus isolated from semi-arid regions were observed only in AD clone libraries. Thus, it was concluded that AD impacts not only airborne fungal concentrations but also fungal communities.

Original language	English
Pages (from-to)	39-50
Number of pages	12
Journal	Atmospheric Environment
Volume	81
DOIs	https://doi.org/10.1016/j.atmosenv.2013.08.039
State	Published - 2013

Bibliographical note

Funding Information:
This study was partly supported by the grants for environmental disease research from the Seoul Metropolitan Government , Republic of Korea, 12-65-010 . The authors thank Dr. Han-Kyu Seo of Bio-Medical Science Co. LTD for many helpful suggestions on the use of the TaqMan system and the design of primers. YP Kim was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0016297 ).

Keywords

Airborne culturable fungal concentration
Allergenic fungi
Asian dust events
ITS clone library
MSQPCR

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10.1016/j.atmosenv.2013.08.039

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@article{cb14b6077fc545efbc7222555e411d47,

title = "Impacts of Asian dust events on atmospheric fungal communities",

abstract = "The composition of atmospheric fungi in Seoul during Asian dust events were assessed by culturing and by molecular methods such as mold specific quantitative PCR (MSQPCR) and internal transcribed spacer cloning (ITS cloning). Culturable fungal concentrations in the air were monitored from May 2008 to July 2011 and 3 pairs of ITS clone libraries, one during Asian dust (AD) day and the other during the adjacent non Asian dust (NAD) day for each pair, were constructed after direct DNA extraction from total suspended particles (TSP) samples. In addition, six aeroallergenic fungi in the atmosphere were also assessed by MSQPCR from October, 2009 to November, 2011. The levels of the airborne culturable fungal concentrations during AD days was significantly higher than that of NAD days (P<0.005). In addition, the correlation of culturable fungal concentrations with particulate matters equal to or less than 10μm in aerodynamic diameter (PM10) concentrations was observed to be high (0.775) for the AD days while correlation coefficients of PM10 as well as other particulate parameters with airborne fungal concentrations were significantly negative for the NAD days during intensive monitoring periods (May to June, 2008). It was found that during AD days several airborne allergenic fungal levels measured with MSQPCR increased up to 5-12 times depending on the species. Comparison of AD vs. NAD clones showed significant differences (P<0.05) in all three cases using libshuff. In addition, high proportions of uncultured soil fungus isolated from semi-arid regions were observed only in AD clone libraries. Thus, it was concluded that AD impacts not only airborne fungal concentrations but also fungal communities.",

keywords = "Airborne culturable fungal concentration, Allergenic fungi, Asian dust events, ITS clone library, MSQPCR",

author = "Jeon, {Eun Mi} and Kim, {Yong Pyo} and Kweon Jeong and Kim, {Ik Soo} and Eom, {Suk Won} and Choi, {Young Zoo} and Ka, {Jong Ok}",

note = "Funding Information: This study was partly supported by the grants for environmental disease research from the Seoul Metropolitan Government , Republic of Korea, 12-65-010 . The authors thank Dr. Han-Kyu Seo of Bio-Medical Science Co. LTD for many helpful suggestions on the use of the TaqMan system and the design of primers. YP Kim was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0016297 ).",

year = "2013",

doi = "10.1016/j.atmosenv.2013.08.039",

language = "English",

volume = "81",

pages = "39--50",

journal = "Atmospheric Environment",

issn = "1352-2310",

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T1 - Impacts of Asian dust events on atmospheric fungal communities

AU - Jeon, Eun Mi

AU - Kim, Yong Pyo

AU - Jeong, Kweon

AU - Kim, Ik Soo

AU - Eom, Suk Won

AU - Choi, Young Zoo

AU - Ka, Jong Ok

N1 - Funding Information: This study was partly supported by the grants for environmental disease research from the Seoul Metropolitan Government , Republic of Korea, 12-65-010 . The authors thank Dr. Han-Kyu Seo of Bio-Medical Science Co. LTD for many helpful suggestions on the use of the TaqMan system and the design of primers. YP Kim was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0016297 ).

PY - 2013

Y1 - 2013

N2 - The composition of atmospheric fungi in Seoul during Asian dust events were assessed by culturing and by molecular methods such as mold specific quantitative PCR (MSQPCR) and internal transcribed spacer cloning (ITS cloning). Culturable fungal concentrations in the air were monitored from May 2008 to July 2011 and 3 pairs of ITS clone libraries, one during Asian dust (AD) day and the other during the adjacent non Asian dust (NAD) day for each pair, were constructed after direct DNA extraction from total suspended particles (TSP) samples. In addition, six aeroallergenic fungi in the atmosphere were also assessed by MSQPCR from October, 2009 to November, 2011. The levels of the airborne culturable fungal concentrations during AD days was significantly higher than that of NAD days (P<0.005). In addition, the correlation of culturable fungal concentrations with particulate matters equal to or less than 10μm in aerodynamic diameter (PM10) concentrations was observed to be high (0.775) for the AD days while correlation coefficients of PM10 as well as other particulate parameters with airborne fungal concentrations were significantly negative for the NAD days during intensive monitoring periods (May to June, 2008). It was found that during AD days several airborne allergenic fungal levels measured with MSQPCR increased up to 5-12 times depending on the species. Comparison of AD vs. NAD clones showed significant differences (P<0.05) in all three cases using libshuff. In addition, high proportions of uncultured soil fungus isolated from semi-arid regions were observed only in AD clone libraries. Thus, it was concluded that AD impacts not only airborne fungal concentrations but also fungal communities.

AB - The composition of atmospheric fungi in Seoul during Asian dust events were assessed by culturing and by molecular methods such as mold specific quantitative PCR (MSQPCR) and internal transcribed spacer cloning (ITS cloning). Culturable fungal concentrations in the air were monitored from May 2008 to July 2011 and 3 pairs of ITS clone libraries, one during Asian dust (AD) day and the other during the adjacent non Asian dust (NAD) day for each pair, were constructed after direct DNA extraction from total suspended particles (TSP) samples. In addition, six aeroallergenic fungi in the atmosphere were also assessed by MSQPCR from October, 2009 to November, 2011. The levels of the airborne culturable fungal concentrations during AD days was significantly higher than that of NAD days (P<0.005). In addition, the correlation of culturable fungal concentrations with particulate matters equal to or less than 10μm in aerodynamic diameter (PM10) concentrations was observed to be high (0.775) for the AD days while correlation coefficients of PM10 as well as other particulate parameters with airborne fungal concentrations were significantly negative for the NAD days during intensive monitoring periods (May to June, 2008). It was found that during AD days several airborne allergenic fungal levels measured with MSQPCR increased up to 5-12 times depending on the species. Comparison of AD vs. NAD clones showed significant differences (P<0.05) in all three cases using libshuff. In addition, high proportions of uncultured soil fungus isolated from semi-arid regions were observed only in AD clone libraries. Thus, it was concluded that AD impacts not only airborne fungal concentrations but also fungal communities.

KW - Airborne culturable fungal concentration

KW - Allergenic fungi

KW - Asian dust events

KW - ITS clone library

KW - MSQPCR

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U2 - 10.1016/j.atmosenv.2013.08.039

DO - 10.1016/j.atmosenv.2013.08.039

M3 - Article

AN - SCOPUS:84884367369

SN - 1352-2310

VL - 81

SP - 39

EP - 50

JO - Atmospheric Environment

JF - Atmospheric Environment

ER -

Impacts of Asian dust events on atmospheric fungal communities

Abstract

Bibliographical note

Keywords

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