Impact of factors affecting the efficacy of intense pulsed light for reducing Bacillus subtilis spores

Hee Jeong Hwang, Gyu A. Kim, Myong Soo Chung

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7 Scopus citations


This study investigated how the following four intense pulsed light (IPL) treatment factors affect the inactivation of Bacillus subtilis (KCCM 11,315) spores: distance between the sample and IPL lamp (8, 13, and 18 cm), pulse width (0.5, 1.3, and 2.1 ms), charging voltage (1000, 1200, and 1400 V), and processing time (10, 20, and 30 s). The results showed that all four factors considerably influenced the spore inactivation rate in different ways. Excluding processing time, which does not affect the pulse itself, the effect was largest for pulse width, followed by distance, and charging voltage. The optimal treatment condition that maximized the inactivation rate was a distance of 8 cm, a pulse width of 2.1 ms, a charging voltage of 1000 V, and a processing time of 30 s, which together produced a 6 log reduction. It revealed that individual factors need to be investigated together for achieving the optimal condition of IPL.

Original languageEnglish
Pages (from-to)1321-1329
Number of pages9
JournalFood Science and Biotechnology
Issue number10
StatePublished - Oct 2021

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A2C1085100).

Publisher Copyright:
© 2021, The Korean Society of Food Science and Technology.


  • Bacillus subtilis
  • Intense pulsed light (IPL)
  • Optimal condition
  • Pulse width
  • Spore


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