Phellinus linteus was examined on its immunostimulating activities using an in vitro immunization and plaque forming cell assay. When lymphocytes were exposed to the extract of Phellinus linteus, the number of antibody forming cell was increased. In in vitro plaque forming cell assay, the immunostimulating effect was about 4.8 and 5.0 times of unimmunized control in polyclonal and T-independent antibody response, respectively. Especially, Phellinus linteus significantly increased the antigenicity of TNP-LPS used as T-independent antigen. But Phellinus linteus did not show a mitogenic effect on B-lymphocytes. These results suggest that immunostimulating activity of Phillinus linteus might be associated with a funtional stimulation of B-lymphocyte involved in humoral immune responses.
- antibody-forming cell polyclonal antigen
- Phellinus linteus
- T-independent antigenic effect