Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis

Amanda Pentecost; Min Ju Kim; Sangmin Jeon; Young Ji Ko; Ick Chan Kwon; Yury Gogotsi; Kwangmeyung Kim; Kara L. Spiller

doi:10.1093/rb/rbz012

Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis

Amanda Pentecost, Min Ju Kim, Sangmin Jeon, Young Ji Ko, Ick Chan Kwon, Yury Gogotsi, Kwangmeyung Kim, Kara L. Spiller

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We previously demonstrated that octadecylamine-functionalized nanodiamond (ND-ODA) and dexamethasone (Dex)-adsorbed ND-ODA (ND-ODA-Dex) promoted anti-inflammatory and pro-regenerative behavior in human macrophages in vitro. In this study, we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice. Following local injection in limbs of mice with collagen type II-induced arthritis, microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA-Dex did not experience bone loss to the levels observed in non-treated arthritic controls. A low dose of ND-ODA and ND-ODA-Dex also reduced macrophage infiltration and expression of pro-inflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control, while a high dose of ND-ODA increased expression of these markers. Overall, these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform, and support the need for an in-depth study, especially with respect to the effects of dose.

Original language	English
Pages (from-to)	163-174
Number of pages	12
Journal	Regenerative Biomaterials
Volume	6
Issue number	3
DOIs	https://doi.org/10.1093/rb/rbz012
State	Published - 1 Jun 2019

Keywords

Biomaterial-cell interaction
Drug delivery
Nanobiomaterials

Access to Document

10.1093/rb/rbz012

Cite this

@article{97436c902a7e4495ba8f03f1496a4a2d,

title = "Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis",

abstract = "We previously demonstrated that octadecylamine-functionalized nanodiamond (ND-ODA) and dexamethasone (Dex)-adsorbed ND-ODA (ND-ODA-Dex) promoted anti-inflammatory and pro-regenerative behavior in human macrophages in vitro. In this study, we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice. Following local injection in limbs of mice with collagen type II-induced arthritis, microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA-Dex did not experience bone loss to the levels observed in non-treated arthritic controls. A low dose of ND-ODA and ND-ODA-Dex also reduced macrophage infiltration and expression of pro-inflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control, while a high dose of ND-ODA increased expression of these markers. Overall, these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform, and support the need for an in-depth study, especially with respect to the effects of dose.",

keywords = "Biomaterial-cell interaction, Drug delivery, Nanobiomaterials",

author = "Amanda Pentecost and Kim, {Min Ju} and Sangmin Jeon and Ko, {Young Ji} and Kwon, {Ick Chan} and Yury Gogotsi and Kwangmeyung Kim and Spiller, {Kara L.}",

note = "Funding Information: Work in the lab of KLS is supported by the National Institutes of Health grant R01 HL130037. A.P. was supported by Whitaker International and David L. Boren fellowships. Publisher Copyright: {\textcopyright} 2019 Oxford University Press. All rights reserved.",

year = "2019",

month = jun,

day = "1",

doi = "10.1093/rb/rbz012",

language = "English",

volume = "6",

pages = "163--174",

journal = "Regenerative Biomaterials",

issn = "2056-3418",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis

AU - Pentecost, Amanda

AU - Kim, Min Ju

AU - Jeon, Sangmin

AU - Ko, Young Ji

AU - Kwon, Ick Chan

AU - Gogotsi, Yury

AU - Kim, Kwangmeyung

AU - Spiller, Kara L.

N1 - Funding Information: Work in the lab of KLS is supported by the National Institutes of Health grant R01 HL130037. A.P. was supported by Whitaker International and David L. Boren fellowships. Publisher Copyright: © 2019 Oxford University Press. All rights reserved.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - We previously demonstrated that octadecylamine-functionalized nanodiamond (ND-ODA) and dexamethasone (Dex)-adsorbed ND-ODA (ND-ODA-Dex) promoted anti-inflammatory and pro-regenerative behavior in human macrophages in vitro. In this study, we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice. Following local injection in limbs of mice with collagen type II-induced arthritis, microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA-Dex did not experience bone loss to the levels observed in non-treated arthritic controls. A low dose of ND-ODA and ND-ODA-Dex also reduced macrophage infiltration and expression of pro-inflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control, while a high dose of ND-ODA increased expression of these markers. Overall, these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform, and support the need for an in-depth study, especially with respect to the effects of dose.

AB - We previously demonstrated that octadecylamine-functionalized nanodiamond (ND-ODA) and dexamethasone (Dex)-adsorbed ND-ODA (ND-ODA-Dex) promoted anti-inflammatory and pro-regenerative behavior in human macrophages in vitro. In this study, we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice. Following local injection in limbs of mice with collagen type II-induced arthritis, microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA-Dex did not experience bone loss to the levels observed in non-treated arthritic controls. A low dose of ND-ODA and ND-ODA-Dex also reduced macrophage infiltration and expression of pro-inflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control, while a high dose of ND-ODA increased expression of these markers. Overall, these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform, and support the need for an in-depth study, especially with respect to the effects of dose.

KW - Biomaterial-cell interaction

KW - Drug delivery

KW - Nanobiomaterials

UR - http://www.scopus.com/inward/record.url?scp=85071148144&partnerID=8YFLogxK

U2 - 10.1093/rb/rbz012

DO - 10.1093/rb/rbz012

M3 - Article

AN - SCOPUS:85071148144

SN - 2056-3418

VL - 6

SP - 163

EP - 174

JO - Regenerative Biomaterials

JF - Regenerative Biomaterials

IS - 3

ER -

Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis

Abstract

Keywords

Access to Document

Fingerprint

Cite this