Immunoliposomes carrying plasmid DNA: preparation and characterization

Na Hyung Kim, Hyo Min Park, Soo Yeon Chung, Eun Jung Go, Hwa Jeong Lee

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The objective of this study was to characterize immunoliposomes carrying plasmid DNA with optimal encapsulation efficiency and antibody density. Plasmid DNA was encapsulated by the freezing/thawing method into liposomes composed of POPC (1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine), DDAB (didodecyl dimethyl ammonium bromide), DSPE-PEG 2000 (dis-tearoyl phosphatidyl ethanolamine polyethylene glycol 2000) and DSPE-PEG 2000-maleimide. The liposomes carrying plasmid DNA were extruded through two stacked polycarbonate filters, of different pore size, to control the liposome size. Then, rat IgG molecules were conjugated to the liposomes. The immunoliposomes containing plasmid DNA were separated from the free plasmid DNA and unconjugated IgG by Sepharose CL-4B column chromatography. The DNA amount encapsulated was affected by DDAB (cationic lipid) concentration, the initial amount of plasmid DNA between 10 μg and 200 μg, the total lipid amount and plasmid DNA size, but not significantly by liposome size. By varying the ratio of DSPE-PEG 2000-maleimide to IgG, the number of IgG molecules per liposome was changed significantly.

Original languageEnglish
Pages (from-to)1263-1269
Number of pages7
JournalArchives of Pharmacal Research
Issue number12
StatePublished - Dec 2004


  • Conjugation
  • Encapsulation
  • IgG
  • Immunoliposome
  • Plasmid DNA


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