Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

Myeung Ju Kim; Yoon Hee Chung; Kyeung Min Joo; Goo Taeg Oh; Jaewoo Kim; Bonghee Lee; Choong Ik Cha

doi:10.1016/j.neulet.2004.07.047

Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

Myeung Ju Kim, Yoon Hee Chung, Kyeung Min Joo, Goo Taeg Oh, Jaewoo Kim, Bonghee Lee, Choong Ik Cha

Department of Life Science

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Nitric oxide (NO) participates in synaptic plasticity, neuronal development, and apoptosis. The involvement of NO and ionic calcium in synaptic plasticity imply that NO may exert an effect on Ca ²⁺ channels. Therefore, we investigated changes in the expressions of calcium channel subunits (Ca _v1.2/α _1C, Ca _v1.3/ α _1D, Ca _v2.1/α _1A, and Ca _v2.2/α _1B) in nNOS knock-out (-/-) (nNOS ^(-/-)) mouse cerebellum using an immunohistochemical approach. We found that the immunoreactivities of the Ca _v1.2 and Ca _v1.3 subunits were reduced in the cell bodies of Purkinje cells in these mice and that the signal of the Ca _v1.2 subunit in neurons and of the Ca _v1.3 subunit in the neuropils of nNOS ^(-/-) mice cerebellar nuclei were significantly down-regulated. We show, for the first time, that prolonged NO deficiency in the cerebellum may affect calcium channel protein expressions, especially, of the Ca _v1.2 and Ca _v1.3 subunits.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Neuroscience Letters
Volume	369
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2004.07.047
State	Published - 7 Oct 2004

Bibliographical note

Funding Information:
This work was supported by grant No. R01-2003-000-10099-0 from the Basic Research Program of the Korea Science & Engineering Foundation. We extend our thanks for Dr. Oh (Genetic Resources Center, KRIBB, Taejon, Korea) for kindly donating the experimental materials.

Keywords

Calcium channel subunit
Cerebellum
Immunohistochemistry
Neuronal NO synthase (nNOS) knock-out (-/-) mice
Nitric oxide (NO)

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title = "Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum",

abstract = "Nitric oxide (NO) participates in synaptic plasticity, neuronal development, and apoptosis. The involvement of NO and ionic calcium in synaptic plasticity imply that NO may exert an effect on Ca 2+ channels. Therefore, we investigated changes in the expressions of calcium channel subunits (Ca v1.2/α 1C, Ca v1.3/ α 1D, Ca v2.1/α 1A, and Ca v2.2/α 1B) in nNOS knock-out (-/-) (nNOS (-/-)) mouse cerebellum using an immunohistochemical approach. We found that the immunoreactivities of the Ca v1.2 and Ca v1.3 subunits were reduced in the cell bodies of Purkinje cells in these mice and that the signal of the Ca v1.2 subunit in neurons and of the Ca v1.3 subunit in the neuropils of nNOS (-/-) mice cerebellar nuclei were significantly down-regulated. We show, for the first time, that prolonged NO deficiency in the cerebellum may affect calcium channel protein expressions, especially, of the Ca v1.2 and Ca v1.3 subunits.",

keywords = "Calcium channel subunit, Cerebellum, Immunohistochemistry, Neuronal NO synthase (nNOS) knock-out (-/-) mice, Nitric oxide (NO)",

author = "Kim, {Myeung Ju} and Chung, {Yoon Hee} and Joo, {Kyeung Min} and Oh, {Goo Taeg} and Jaewoo Kim and Bonghee Lee and Cha, {Choong Ik}",

note = "Funding Information: This work was supported by grant No. R01-2003-000-10099-0 from the Basic Research Program of the Korea Science & Engineering Foundation. We extend our thanks for Dr. Oh (Genetic Resources Center, KRIBB, Taejon, Korea) for kindly donating the experimental materials.",

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AU - Kim, Myeung Ju

AU - Chung, Yoon Hee

AU - Joo, Kyeung Min

AU - Oh, Goo Taeg

AU - Kim, Jaewoo

AU - Lee, Bonghee

AU - Cha, Choong Ik

N1 - Funding Information: This work was supported by grant No. R01-2003-000-10099-0 from the Basic Research Program of the Korea Science & Engineering Foundation. We extend our thanks for Dr. Oh (Genetic Resources Center, KRIBB, Taejon, Korea) for kindly donating the experimental materials.

PY - 2004/10/7

Y1 - 2004/10/7

N2 - Nitric oxide (NO) participates in synaptic plasticity, neuronal development, and apoptosis. The involvement of NO and ionic calcium in synaptic plasticity imply that NO may exert an effect on Ca 2+ channels. Therefore, we investigated changes in the expressions of calcium channel subunits (Ca v1.2/α 1C, Ca v1.3/ α 1D, Ca v2.1/α 1A, and Ca v2.2/α 1B) in nNOS knock-out (-/-) (nNOS (-/-)) mouse cerebellum using an immunohistochemical approach. We found that the immunoreactivities of the Ca v1.2 and Ca v1.3 subunits were reduced in the cell bodies of Purkinje cells in these mice and that the signal of the Ca v1.2 subunit in neurons and of the Ca v1.3 subunit in the neuropils of nNOS (-/-) mice cerebellar nuclei were significantly down-regulated. We show, for the first time, that prolonged NO deficiency in the cerebellum may affect calcium channel protein expressions, especially, of the Ca v1.2 and Ca v1.3 subunits.

AB - Nitric oxide (NO) participates in synaptic plasticity, neuronal development, and apoptosis. The involvement of NO and ionic calcium in synaptic plasticity imply that NO may exert an effect on Ca 2+ channels. Therefore, we investigated changes in the expressions of calcium channel subunits (Ca v1.2/α 1C, Ca v1.3/ α 1D, Ca v2.1/α 1A, and Ca v2.2/α 1B) in nNOS knock-out (-/-) (nNOS (-/-)) mouse cerebellum using an immunohistochemical approach. We found that the immunoreactivities of the Ca v1.2 and Ca v1.3 subunits were reduced in the cell bodies of Purkinje cells in these mice and that the signal of the Ca v1.2 subunit in neurons and of the Ca v1.3 subunit in the neuropils of nNOS (-/-) mice cerebellar nuclei were significantly down-regulated. We show, for the first time, that prolonged NO deficiency in the cerebellum may affect calcium channel protein expressions, especially, of the Ca v1.2 and Ca v1.3 subunits.

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KW - Cerebellum

KW - Immunohistochemistry

KW - Neuronal NO synthase (nNOS) knock-out (-/-) mice

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Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

Abstract

Bibliographical note

Keywords

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