Immunocytochemical localization of neuronal and inducible nitric oxide synthase in the retina of zebrafish, Brachydanio rerio

Dong Hoon Shin, Hyoung Soo Lim, Sung Kyun Cho, Hwa Young Lee, Hye Won Lee, Kyung Hoon Lee, Yoon Hee Chung, Sa Sun Cho, Choong Ik Cha, Douk Ho Hwang

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Several previous studies have revealed the distribution of neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) in the retina of various species. However, nothing has been reported on the nNOS and iNOS expression in zebrafish retina, even though it has been used as an effective model for developmental studies. In this study on nNOS and iNOS immunoreactivity (IR) in the zebrafish retina, iNOS-IR signals were detected in the ganglion cell layer (GC), the inner plexiform layer (IP), the outer plexiform layer (OP) and the photoreceptor layer (PR). nNOS-IR signals were also intensely detected in the GC, IP, OP and PR. These signals were very similar to the iNOS-IR signals, except for some minor differences in relative intensities. This could be explained by the speculation that the synthesis of nitric oxide by iNOS may represent a compensatory mechanism in the absence of nNOS. We described for the first time the distribution of nNOS and iNOS-IR cells in zebrafish retina. This could provide the basis for further study on the nNOS and iNOS properties of zebrafish retina. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)220-222
Number of pages3
JournalNeuroscience Letters
Volume292
Issue number3
DOIs
StatePublished - 13 Oct 2000

Keywords

  • Immunocytochemistry
  • Inducible nitric oxide synthase
  • Neuronal nitric oxide synthase
  • Retina
  • Zebrafish

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