Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes

H. J. Yang; J. Y. Chung; D. H. Yun; Y. Kong; A. Ito; L. Ma; Y. H. Liu; S. C. Lee; S. Y. Kang; S. Y. Cho

doi:10.1046/j.1365-3024.1998.00179.x

Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes

H. J. Yang, J. Y. Chung, D. H. Yun, Y. Kong, A. Ito, L. Ma, Y. H. Liu, S. C. Lee, S. Y. Kang, S. Y. Cho

Department of Parasitology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was evaluated. Immunoblot analysis revealed that most sera from patients with neurocysticercosis recognized the 10kDa subunit strongly (209/217 cases, 84.6%), while a few sera from individuals with other parasitic diseases including alveolar echinococcosis (AE, 2/20, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions. Sera of cases with other parasitic diseases, especially AE and CE, exhibited cross reactions against other bands in CF. Both differential immunoblot and immunoprecipitation analyses showed that the 10kDa subunit was the most specific to cysticercosis and highly antigenic, whereas other components at 20-40, 64, 95 and 106kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgG1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.

Original language	English
Pages (from-to)	483-488
Number of pages	6
Journal	Parasite Immunology
Volume	20
Issue number	10
DOIs	https://doi.org/10.1046/j.1365-3024.1998.00179.x
State	Published - 1998

Keywords

Alveolar echinococcosis
Cyst fluid antigen
Cystic echinococcosis
Cysticercosis
Serodiagnosis
Taenia solium

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10.1046/j.1365-3024.1998.00179.x

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title = "Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes",

abstract = "The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was evaluated. Immunoblot analysis revealed that most sera from patients with neurocysticercosis recognized the 10kDa subunit strongly (209/217 cases, 84.6%), while a few sera from individuals with other parasitic diseases including alveolar echinococcosis (AE, 2/20, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions. Sera of cases with other parasitic diseases, especially AE and CE, exhibited cross reactions against other bands in CF. Both differential immunoblot and immunoprecipitation analyses showed that the 10kDa subunit was the most specific to cysticercosis and highly antigenic, whereas other components at 20-40, 64, 95 and 106kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgG1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.",

keywords = "Alveolar echinococcosis, Cyst fluid antigen, Cystic echinococcosis, Cysticercosis, Serodiagnosis, Taenia solium",

author = "Yang, {H. J.} and Chung, {J. Y.} and Yun, {D. H.} and Y. Kong and A. Ito and L. Ma and Liu, {Y. H.} and Lee, {S. C.} and Kang, {S. Y.} and Cho, {S. Y.}",

year = "1998",

doi = "10.1046/j.1365-3024.1998.00179.x",

language = "English",

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AU - Yang, H. J.

AU - Chung, J. Y.

AU - Yun, D. H.

AU - Kong, Y.

AU - Ito, A.

AU - Ma, L.

AU - Liu, Y. H.

AU - Lee, S. C.

AU - Kang, S. Y.

AU - Cho, S. Y.

PY - 1998

Y1 - 1998

N2 - The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was evaluated. Immunoblot analysis revealed that most sera from patients with neurocysticercosis recognized the 10kDa subunit strongly (209/217 cases, 84.6%), while a few sera from individuals with other parasitic diseases including alveolar echinococcosis (AE, 2/20, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions. Sera of cases with other parasitic diseases, especially AE and CE, exhibited cross reactions against other bands in CF. Both differential immunoblot and immunoprecipitation analyses showed that the 10kDa subunit was the most specific to cysticercosis and highly antigenic, whereas other components at 20-40, 64, 95 and 106kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgG1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.

AB - The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was evaluated. Immunoblot analysis revealed that most sera from patients with neurocysticercosis recognized the 10kDa subunit strongly (209/217 cases, 84.6%), while a few sera from individuals with other parasitic diseases including alveolar echinococcosis (AE, 2/20, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions. Sera of cases with other parasitic diseases, especially AE and CE, exhibited cross reactions against other bands in CF. Both differential immunoblot and immunoprecipitation analyses showed that the 10kDa subunit was the most specific to cysticercosis and highly antigenic, whereas other components at 20-40, 64, 95 and 106kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgG1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.

KW - Alveolar echinococcosis

KW - Cyst fluid antigen

KW - Cystic echinococcosis

KW - Cysticercosis

KW - Serodiagnosis

KW - Taenia solium

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DO - 10.1046/j.1365-3024.1998.00179.x

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SN - 0141-9838

VL - 20

SP - 483

EP - 488

JO - Parasite Immunology

JF - Parasite Immunology

IS - 10

ER -

Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes

Abstract

Keywords

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