IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells

Beom Su Kim, Hyung Jin Kim, Jin Seong Kim, Yong Ouk You, Homa Zadeh, Hong In Shin, Seung Jin Lee, Yoon Jeong Park, Takashi Takata, Sung Hee Pi, Jun Lee, Hyung Keun You

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The exact molecular mechanisms governing the differentiation of bone marrow stromal stem/progenitor cells (BMSCs) into osteoblasts remain largely unknown. In this study, a highly expressed protein that had a high degree of homology with interferon-induced transmembrane protein 1 (IFITM1) was identified using differentially expressed gene (DEG) screening. We sought to determine whether IFITM1 influenced osteoblast differentiation. During differentiation, IFITM1 expression gradually increased from 5 to 10. days and subsequently decreased at 15. days in culture. Analysis of IFITM1 protein expression in several cell lines as well as in situ studies on human tissues revealed its selective expression in bone cells and human bone. Proliferation of human alveolar-derived bone marrow stromal cells (hAD-BMSCs) was significantly inhibited by IFITM1 knockdown by using short hairpin RNA, as were bone specific markers such as alkaline phosphatase, collagen type I α 1, bone sialoprotein, osteocalcin, and osterix were decreased. Calcium accumulation also decreased following IFITM1 knockdown. Moreover, IFITM1 knockdown in hAD-BMSCs was associated with inhibition of Runx2 mRNA and protein expression. Collectively, the present data provide evidence for the role of IFITM1 in osteoblast differentiation. The exact mechanisms of IFITM1's involvement in osteoblast differentiation are still under investigation.

Original languageEnglish
Pages (from-to)506-514
Number of pages9
JournalBone
Volume51
Issue number3
DOIs
StatePublished - Sep 2012

Keywords

  • Alveolar bone marrow
  • IFITM1
  • Mesenchymal stromal cells
  • Osteoblast differentiation
  • Runx2

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