TY - JOUR
T1 - Identification of up-regulated genes in malignant glioma with subtraction hybridization
T2 - Preliminary screening studies
AU - Cho, Yong Jae
PY - 2008/4
Y1 - 2008/4
N2 - Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database. Results: Using semi-quantitative reverse transription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
AB - Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database. Results: Using semi-quantitative reverse transription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
KW - Gene
KW - Glioblastoma
KW - Subtraction hybridization
UR - http://www.scopus.com/inward/record.url?scp=44649119458&partnerID=8YFLogxK
U2 - 10.3349/ymj.2008.49.2.301
DO - 10.3349/ymj.2008.49.2.301
M3 - Article
C2 - 18452269
AN - SCOPUS:44649119458
SN - 0513-5796
VL - 49
SP - 301
EP - 310
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
IS - 2
ER -