Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system

Taesook Yoon, Jaehoon Jung, Minjeong Kim, Kang Man Lee, Eung Chil Choi, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

To further understand the biological function of translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor (HRF), the yeast two-hybrid system was used to screen interacting molecules. We isolated cDNA clones coding for TCTP/HRF, suggesting that it may have a self-interacting property. Domain mapping of the interaction revealed that the C-terminal region of residue 126-172 is involved in self-interaction. The self-interacting property of TCTP/HRF was further supported by FPLC gel-filtration chromatography and coimmunoprecipitation analysis from transfected COS-7 cells. Our data suggests that TCTP/HRF may have a potential to self-interact through the C-terminal region, and the self-interaction property may be related to its biological function.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume384
Issue number2
DOIs
StatePublished - 1 Jan 2000

Keywords

  • Coimmunoprecipitation
  • FPLC
  • Self-interaction
  • Translationally controlled tumor protein
  • Yeast two-hybrid

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