Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system

Taesook Yoon, Jaehoon Jung, Minjeong Kim, Kang Man Lee, Eung Chil Choi, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

To further understand the biological function of translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor (HRF), the yeast two-hybrid system was used to screen interacting molecules. We isolated cDNA clones coding for TCTP/HRF, suggesting that it may have a self-interacting property. Domain mapping of the interaction revealed that the C-terminal region of residue 126-172 is involved in self-interaction. The self-interacting property of TCTP/HRF was further supported by FPLC gel-filtration chromatography and coimmunoprecipitation analysis from transfected COS-7 cells. Our data suggests that TCTP/HRF may have a potential to self-interact through the C-terminal region, and the self-interaction property may be related to its biological function.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume384
Issue number2
DOIs
StatePublished - 1 Jan 2000

Bibliographical note

Funding Information:
We thank Dr. Jongsun Kim for previewing the manuscript and Dr. Man Wook Hur for providing us TCTP/HRF polyclonal antibody. This work was supported by KOSEF through CCSR at Ewha Woman’s University, KISTEP research fund for Women’s University, and the Korean Ministry of Education. T. Yoon was supported by the Brain Korea 21 Project fellowship.

Keywords

  • Coimmunoprecipitation
  • FPLC
  • Self-interaction
  • Translationally controlled tumor protein
  • Yeast two-hybrid

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