Identification of the Calcium Binding Sites in Translationally Controlled Tumor Protein

Moonhee Kim, Yoonwha Jung, Kyunglim Lee, Choonmi Kim

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor, is a growth-related tumor protein. Although the primary sequence of rat TCTP does not reveal any recognizable Ca2+ -binding motif, previous studies have demonstrated that rat TCTP consisting of 172 amino acids is a Ca2+ -binding protein. However, the region of TCTP required for Ca2+ interaction has not been mapped to the molecule. Here, we reported that the Ca2+ binding region of TCTP, which was mapped by using a combination of deletion constructs of rat TCTP and 45Ca2+-overlay assay, was confined to amino acid residues 81-112. This binding domain did not show any peculiar loop of calcium-binding motif such as CaLB domain and EF hand motif and it seems to be constituted of random coil regions neighboring the a helix. Thus, our data confirm that TCTP is a novel family of Ca2+-binding protein.

Original languageEnglish
Pages (from-to)633-636
Number of pages4
JournalArchives of Pharmacal Research
Volume23
Issue number6
DOIs
StatePublished - 2000

Keywords

  • Calcium-overlay assay
  • Calcium-binding sites
  • TCTP

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