Identification of radiation-specific responses from gene expression profile

Woong Yang Park, Chang Il Hwang, Chang Nim Im, Min Ji Kang, Jang Hee Woo, Ju Hoon Kim, Yon Su Kim, Ju Han Kim, Ho Kim, Kyung A. Kim, Hyung Jin Yu, Sue Jae Lee, Yun Sil Lee, Jeong Sun Seo

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in γ-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make 'RadChip'. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon γ-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cγ2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after γ-radiation in RT-PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-κB upon γ-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.

Original languageEnglish
Pages (from-to)8521-8528
Number of pages8
JournalOncogene
Volume21
Issue number55
DOIs
StatePublished - 5 Dec 2002

Keywords

  • γ-irradiation
  • CDNA microarray
  • Cytoplasmic epoxide hydrolase
  • P53
  • Phospholipase Cγ2

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