TY - JOUR
T1 - Identification of New Polyacetylenes from Dendropanax morbifera with PPAR-α Activity Study
AU - Piao, Donglan
AU - Youn, Isoo
AU - Huynh, Thanh Hau
AU - Kim, Hyun Woo
AU - Noh, Sang Gyun
AU - Chung, Hae Young
AU - Oh, Dong Chan
AU - Seo, Eun Kyoung
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/12
Y1 - 2024/12
N2 - Dendropanax morbifera Leveille is a traditional medicine used to treat migraine headache and dysmenorrhea. In this study, three polyacetylenes, methyl (10E,9R,16R)-16-acetoxy-9-hydroxyoctadeca-10,17-dien-12,14-diynoate (1), methyl (10E,9R,16S)-9,16-dihydroxyoctadeca-10-en-12,14-diynoate (2), and methyl (10Z,9R,16S)-9,16-dihydroxyoctadeca-10,17-dien-12,14-diynoate (3), were isolated from the aerial parts of D. morbifera, together with seven known compounds (4–10). Importantly, the isolates (6 and 8) were found in the family Araliaceae for the first time in this study. Compounds 1−10 were evaluated for their binding affinity to AMPK and CTSS receptors using in silico docking simulations. Only compound 7 increased the protein expression levels of PPAR-α, Sirt1, and AMPK when administered to HepG2 cells as a PPAR-α agonist. On the other hand, 7 did not produce any significant reduction in CTSS activity. This study could pave the way for the discovery of novel treatments from D. morbifera targeting PPAR-α and AMPK.
AB - Dendropanax morbifera Leveille is a traditional medicine used to treat migraine headache and dysmenorrhea. In this study, three polyacetylenes, methyl (10E,9R,16R)-16-acetoxy-9-hydroxyoctadeca-10,17-dien-12,14-diynoate (1), methyl (10E,9R,16S)-9,16-dihydroxyoctadeca-10-en-12,14-diynoate (2), and methyl (10Z,9R,16S)-9,16-dihydroxyoctadeca-10,17-dien-12,14-diynoate (3), were isolated from the aerial parts of D. morbifera, together with seven known compounds (4–10). Importantly, the isolates (6 and 8) were found in the family Araliaceae for the first time in this study. Compounds 1−10 were evaluated for their binding affinity to AMPK and CTSS receptors using in silico docking simulations. Only compound 7 increased the protein expression levels of PPAR-α, Sirt1, and AMPK when administered to HepG2 cells as a PPAR-α agonist. On the other hand, 7 did not produce any significant reduction in CTSS activity. This study could pave the way for the discovery of novel treatments from D. morbifera targeting PPAR-α and AMPK.
KW - AMP-activated protein kinase
KW - cathepsin S
KW - Dendropanax morbifera Leveille
KW - Peroxisome proliferator-activated receptor
KW - polyacetylene
UR - http://www.scopus.com/inward/record.url?scp=85213292318&partnerID=8YFLogxK
U2 - 10.3390/molecules29245942
DO - 10.3390/molecules29245942
M3 - Article
C2 - 39770031
AN - SCOPUS:85213292318
SN - 1420-3049
VL - 29
JO - Molecules
JF - Molecules
IS - 24
M1 - 5942
ER -